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We narrowed to 21,525 results for: SPR

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Showing: 1361 - 1400 of 21525 results

  7. PB-PE SpRY_ATH51

    Plasmid
    #235998
    Purpose
    Expresses SpRY_ATH51 prime editor and pegRNA in mammalian cells
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    SpRY_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    bpSV40 NLS
    Expression
    Mammalian
    Mutation
    ectopic insertion of 7 amino acids of PID turn-he…
    Available Since
    Aug. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    235998-plasmid-map-sequence-id-470119

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  8. pLentiCRISPR_mCherry

    Plasmid
    #229078
    Purpose
    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1s
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    229078-plasmid-map-sequence-id-456127

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  18. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Article
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  20. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  23. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid
    #70657
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against DDX3Y
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  29. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Larry Gerace
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175136-plasmid-map-sequence-id-352023

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Showing: 1361 - 1400 of 21525 results