162,300 results
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Plasmid#227325PurposeDonor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)DepositorInsertGOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
5XUAS IVS CsChrimson tdtomato_tr
Plasmid#111545PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertCsChrimson_tdtomato_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn-fDIO-taCasp3-TEVp
Plasmid#207997PurposeInducing apoptotic ablation of neurons through a process reliant on Flp recombinaseDepositorInserttaCasp3-TEVp
UseAAVTagsExpressionMutationPromoterhSyn promoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
PL452-hGH-polyA-PGK-neoR
Plasmid#191681PurposeDonor plasmid backbone PGK-neoRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc
Plasmid#16539Purposeluciferase reporter plasmid with very low basal activityDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport RXR alpha
Plasmid#8882DepositorInsertRXR alpha (RXRA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
3CEG
Plasmid#60107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBIRC6:SDC136-F10:C41228 (BIRC6 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2/pAAV
Plasmid#91718PurposeAAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKIIDepositorInsertmEGFP-P2A-paAIP2
UseAAVTagsExpressionMutationPromoterCaMKII 1.3Available sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1782
Plasmid#143315PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF239 (ZNF239 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1200
Plasmid#138526PurposeFor expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.DepositorUseTagsN-terminal His tag and TEV cleavage site on MlaDExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SCP1-pUC119
Plasmid#21846DepositorInsertSCP1 (super core promoter 1)-pUC119
UsePuc derivative - a cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE
Plasmid#203847PurposeFlp-dependent expression of Chronos fused to EGFPDepositorInsertChronos
UseAAVTagsEGFPExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB301-2μ-HDV
Plasmid#196329PurposeA yeast-Escherichia coli-Agrobacterium shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3527
Plasmid#145003PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertERF (ERF Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-IDH1
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorInsertisocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Kl-EGFP
Plasmid#45532DepositorInsertalpha Klotho (Kl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorInsertR-spondin 1 (RSPO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only