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pIntegrase_9

Plasmid

#60580

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_9

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Ptgs2

Plasmid

#209519

Purpose

Expresses luciferase under control of Ptgs2 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Ptgs2 (Ptgs2 Mouse)

Use

Luciferase

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDN-Sth1Cas9

Plasmid

#221386

Purpose

Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

Sth1 Cas9

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDB5322

Plasmid

#204832

Purpose

An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW4 promoter

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK562 (pDS-GFP)

Plasmid

#183129

Purpose

Control plasmid that expresses off-target dsRNA

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

GFPmut3 (no RBS)

Use

Synthetic Biology

Available Since

July 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

UAS

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP35_MARV

Plasmid

#135471

Purpose

Encodes Marburg virus VP35 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

Insert

VP35

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pVH607-Tier1-PCREm4-Nluc

Plasmid

#169594

Purpose

Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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sReach-mTurquoise2

Plasmid

#98840

Purpose

Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

sReach-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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G555

Plasmid

#216483

Purpose

Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

Depositor

Christophe Danelon

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

plsB, plsC, cdsA, pssA

Use

Cell-free expression

Promoter

T7 for each gene

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKB01_mOrange2

Plasmid

#69102

Purpose

Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mOrange2

Use

Synthetic Biology

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry

Plasmid

#221216

Purpose

control plasmid-part B of SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

NLS-mCherry

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRBBm34

Plasmid

#48114

Purpose

Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pRnc-gGFP161

Plasmid

#189536

Purpose

Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

Depositor

Marc Ostermeier

Article

Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

Inserts

RNase III
gUTR-161-GFP

Expression

Bacterial

Mutation

N/A

Promoter

J23119 and pBAD

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

Plasmid

#176575

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP L138 Reporter

Plasmid

#119130

Purpose

Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

mCherry and eGFP

Use

Lentiviral

Mutation

eGFP L138S

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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