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Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-2uDelta13
Plasmid#12990DepositorInsertFLIPglu F16F D152A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationD152A mutation in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-empty
Plasmid#221239PurposeExpression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transductionDepositorInsertFLAG-4xFKBP scaffold
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC15-P1B3
Plasmid#202856PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15DepositorInsertEGFP/BC15
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC11-P1A11
Plasmid#202852PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11DepositorInsertEGFP/BC11
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-600uDelta13
Plasmid#12991DepositorInsertFLIPglu F16A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationF16A in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-apraR
Plasmid#199993PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraADepositorInsertT7RNAP Circuit
UseTagsExpressionBacterialMutationcopFnullPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-PARG shRNA
Plasmid#23260DepositorInsertpoly(ADP-ribose) glycohydrolase shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromotermin pCYC1Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ntH6-eGFP
Plasmid#32557DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pER-eZinCh-2
Plasmid#70009Purposeexpresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cellsDepositorInsertER-eZinCh-2
UseTagsKDEL sequence and PPI signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PKCG
Plasmid#16180DepositorInsertPRKC gamma (PRKCG Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCas5
Plasmid#82378PurposesgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pML3009
Plasmid#193256PurposeGamma-aminobutyric acid sensor expressing eYFP reporterDepositorInsertsgabR
eYFP
UseSynthetic BiologyTagsExpressionMutationPromotersynthetic promoter P2 and synthetic promoter PGab…Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB8792
Plasmid#126910PurposeTemplate for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 geneDepositorInsertADE2 gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC82-P1G10
Plasmid#202923PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82DepositorInsertEGFP/BC82
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCLR2070
Plasmid#28063DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationContains N496D point mutation in FokI domain, as …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCLR2068
Plasmid#28062DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationContains H537R point mutation in FokI domain, as …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC2-P1A2
Plasmid#202843PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2DepositorInsertEGFP/BC2
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mMaple3
Plasmid#154912PurposeExpresses human PFAS-mMaple3 fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseTagsmMaple3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAN101
Plasmid#131123PurposePlasmid for overexpression of prion domains to induce prionsDepositorTypeEmpty backboneUseTagsmKate2ExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K840R
Plasmid#204418PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
UseTagsHA and YFPExpressionMammalianMutationLysine 840 to Arginine (K840R)PromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K1097R
Plasmid#204417PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
UseTagsHA and YFPExpressionMammalianMutationLysine 1097 to Arginine (K1097R)PromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC6-P1A6
Plasmid#202847PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6DepositorInsertEGFP/BC6
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB5191
Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b(K72A)
Plasmid#23258DepositorInsertDNA polymerase beta (K72A) (POLB Human)
UseTagsFlagExpressionMammalianMutationK72APromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27NPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_WTTS
Plasmid#91233Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Mtb-TF
Plasmid#111513PurposeExpresses M. tuberculosis Trigger Factor with a C-terminal His tagDepositorInsertTrigger Factor
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ptet-eGFP
Plasmid#32554DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterptetAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pBAD-eGFP
Plasmid#32553DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterpBADAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR-A2BP1
Plasmid#16176DepositorInsertAtaxin2-binding protein 1 (RBFOX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOIMars1CyN
Plasmid#75221PurposeMammalian cell surface expression of fluorogen activating protein Mars1CyDepositorInsertss-HA-Mars1Cy-CD80(237-306)
UseTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only