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19,779 results

Showing: 10771 - 10800 of 19779 results

  5. VIPR2-Tango

    Plasmid
    #66521
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    VIPR2 (VIPR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  7. pcDNA3.3_CoV2_P1

    Plasmid
    #170450
    Purpose
    Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
    Tags
    NA
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Promoter
    CMV
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170450-plasmid-map-sequence-id-333035

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  13. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Article
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
    Use
    Shuttle vector for insect expression vectors
    Mutation
    codons altered for Drosophila and to eliminate re…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51814-plasmid-map-sequence-id-79554

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  27. pAluYa5-neo-TET

    Plasmid
    #51283
    Purpose
    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    AluYa5
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    upstream pol III enhancer sequence of the 7SL gen…
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51283-plasmid-map-sequence-id-78362

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  29. pSBbi-GN

    Plasmid
    #60517
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60517-plasmid-map-sequence-id-379976

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Showing: 10771 - 10800 of 19779 results