20,147 results
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Plasmid#105729PurposeMammalian expression of mutant human alpha synucleinDepositorAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET28-hXRCC1
Plasmid#70759Purposebacterial expression of human XRCC1DepositorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSico p53
Plasmid#12089PurposeCre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression.DepositorInsertp53 shRNA (Trp53 Mouse)
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP
Plasmid#52675PurposeshRNA silencing plasmid with eGFP transfection markerDepositorTypeEmpty backboneUseRNAiTagsCMV-EGFPPromoterCMV and U6Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
FFA1-Tango
Plasmid#66280PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-Zif268
Plasmid#12610DepositorInsertZif268 binding site in pH3U3
ExpressionBacterialAvailable SinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
psd44-G13QL
Plasmid#46828Purposeexpression of mutant G protein alpha 13 in mammalian cellsDepositorInsertGNA13 (GNA13 Human)
UseLentiviralExpressionMammalianMutationQ226L (The mutation reduces GTPase activity resul…PromoterPGK promoterAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPA6
Plasmid#19486DepositorAvailable SinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N
Plasmid#80930PurposeExpresses Cas9N in mammalian cells.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Drp1
Plasmid#141159PurposeMammalian expression and labeling of Drp1DepositorAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AK1-His
Plasmid#124135PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
ExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184V
Plasmid#106175PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184VPromoterhSynapsinAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP
Plasmid#165830PurposeEGFP green fluorescent protein CDSDepositorInsertEGFP
ExpressionInsectAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-1xms2-2x3’UTR
Plasmid#122946PurposeFor expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTRDepositorInsertSaCas9-MS2-HBB 3' UTR
UseAAVExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
DDB1
Plasmid#25246PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDDB1_1 (DDB1 Human)
UseBaculovirus expressionTagsHisExpressionInsectPromoterpolyhedrinAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET_RTX(exo-)-6xHis
Plasmid#145028PurposepET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tagDepositorArticleInsertRTX(exo-)
Tags6xHisExpressionBacterialMutationN210DPromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Actin-C-18
Plasmid#54967PurposeLocalization: Actin, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterEFSAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-4SS-HEWL
Plasmid#39233DepositorInsertHen egg white lysozyme (LYZ Chicken)
ExpressionBacterialMutationN-terminal MetPromoterT7Available SinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS1
Plasmid#196977PurposeExpresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS1 tag
ExpressionPlantPromoterpG10-90Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLM-vexGFP-Oct4
Plasmid#22240Purpose2nd generation lentiviral vectorDepositorInsertvexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…Available SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
p40PX-EGFP
Plasmid#19010DepositorInsertp40phox (NCF4 Human)
TagsEGFPExpressionMammalianMutationaa1-148 (PX domains)PromoterCMVAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
mIFP-N1
Plasmid#54620PurposeLocalization: N1 Cloning Vector, Excitation: 683, Emission: 704DepositorTypeEmpty backboneTagsmIFPExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
LV-Sox10MCS5-GFP
Plasmid#115783PurposeThis plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.DepositorInsertmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
UseLentiviralTagscfos basal promoter conjugated MCS5 promoter fuse…PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDH2-WT
Plasmid#87926PurposeExpresses IDH2 (wild-type) in mammalian cellsDepositorInsertisocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
TagsnoneExpressionMammalianPromoterCMVAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only