We narrowed to 11,283 results for: Vars;

1. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

   Plasmid

   #111388

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. plx307 SHOC2 V5 WT

   Plasmid

   #190568

   Purpose

   SHOC2 variant overexpression

   Depositor

   William Hahn

   Article

   Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

   Insert

   SHOC2 (SHOC2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2R3a-VenusYFP-3AT

   Plasmid

   #71269

   Purpose

   Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   VenusYFP
   

   Use

   Gateway

   Tags

   4xGly linker and T3A pea Pisum sativum ribulose-1…

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1α1.1-tdTomato

   Plasmid

   #122101

   Purpose

   AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Promoter

   EF1a（1.1kb short version)

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH733-2µ-RLuc/maxCFLuc

   Plasmid

   #40608

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

   Plasmid

   #212487

   Purpose

   Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR1A mKate2-hu_ncOGT K842M

   Plasmid

   #154284

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   K842M mutation (Catalytic Inactive)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-ITR-PytR(PyOtR)-mCherry

   Plasmid

   #204870

   Purpose

   Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   M. mazei pyrrolysyl tRNA mutant "PyOtR"
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

   Promoter

   U6

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX304-GFP-v1 IQSEC1 WT

   Plasmid

   #162016

   Purpose

   Expression of IQSEC1 splice variant 1

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   IQSEC1 (IQSEC1 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid

    #122681

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 153 to Alanine

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX304-GFP-v2 IQSEC1 WT

    Plasmid

    #162017

    Purpose

    Expression of IQSEC1 splice variant 2

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pαH-S-HRV3C

    Plasmid

    #164567

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike - Furin site changed for HRV3C site
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    682-685 (furin site) replaced with HRV3C site (LE…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid

    #155013

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    A227Y

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

    Plasmid

    #69051

    Purpose

    lentiviral expression of IKZF1 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    UBC

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX304-GFP-v3 IQSEC1 WT

    Plasmid

    #162018

    Purpose

    Expression of IQSEC1 splice variant 3

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX304-GFP-v4 IQSEC1 WT

    Plasmid

    #162019

    Purpose

    Expression of IQSEC1 splice variant 4

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN-T2dMFot

    Plasmid

    #44558

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    rtetR-M2::FFF
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

    Promoter

    PTETREG

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR1A mKate2-hu_ncOGT D554N

    Plasmid

    #154283

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    D554N mutation (Ser/Thr Glycosylation Impaired)

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3322_pETcon_SARS2_Omicron-BA2

    Plasmid

    #184409

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid

    #154285

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations (HCF-…

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. P2_HNF4A8

    Plasmid

    #31094

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 8 derived from promoter P2

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

    Plasmid

    #111387

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114099

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

    Plasmid

    #101177

    Purpose

    Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. FR_HNF4A2-C106R

    Plasmid

    #31123

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2, mutation C106R. Wild type seq…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pFLAG-EEF1D (pcDNA 3.1+) LG3-1

    Plasmid

    #37364

    Depositor

    David Litchfield

    Article

    Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

    Insert

    EEF1D (EEF1D Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

28. CMV-218_HNF4A2

    Plasmid

    #31090

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 2 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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29. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-138_HNF4A8

    Plasmid

    #31093

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 8 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge