We narrowed to 17,810 results for: SHE

1. pDONR223_HcRed

   Plasmid

   #25892

   Purpose

   Gateway entry vector containing HcRed.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   hcRed
   

   Use

   Gateway entry vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_Actb HR donor

   Plasmid

   #97317

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC-HYG-YR

   Plasmid

   #61765

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Hygromycin
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Promoter

   trpC promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-ITFG2

   Plasmid

   #87045

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. shTBX5 # 2

   Plasmid

   #42550

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shTBX5 (TBX5 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

6. shTBX5 # 1

   Plasmid

   #42549

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shTBX5 (TBX5 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

7. XE120 CAMKII-K42M 1-271-CS2+

   Plasmid

   #16734

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-K42M 1-271 (Camk2a Rat)
   

   Use

   Xenopus expression

   Mutation

   C-terminal truncation of K42M kinase

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pDONR223-VPS35

   Plasmid

   #21689

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Vacuolar Protein Sorting 35 homolog (VPS35 Human)
   

   Use

   Gateway entry clone

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pRK5 CASTOR1-Flag Cterm

   Plasmid

   #84488

   Purpose

   overexpression of CASTOR1 with Flag tag

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 (GATSL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

10. p11d-tRPA-32Ala9

    Plasmid

    #102616

    Purpose

    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

    Depositor

    Marc Wold

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Expression

    Bacterial

    Mutation

    with an alanine substituted phosphorylation domain

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKII-SomArchon

    Plasmid

    #126942

    Purpose

    Expression of SomArchon under CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSOMA-NLS

    Plasmid

    #118937

    Purpose

    Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Fz4-GFP

    Plasmid

    #42197

    Depositor

    Robert Lefkowitz, Jeremy Nathans

    Article

    Chen et al Science. 2003 Sep 5;301(5638):1391-4.

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSOMA-NES

    Plasmid

    #118939

    Purpose

    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDL14

    Plasmid

    #106901

    Purpose

    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Inserts

    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    

    Expression

    Bacterial

    Mutation

    Lysine 622 was replaced by Alanine

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-mouse p19ARF-IRES-GFP

    Plasmid

    #107147

    Purpose

    Mammalian expression of mouse Arf

    Depositor

    Martine Roussel

    Article

    Kuo et al Cancer Res. 2003 Mar 1;63(5):1046-53.

    Insert

    Arf [alternative reading frame] (Cdkn2a Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-hChR2(E123A)-mCherry

    Plasmid

    #35506

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. hP2X3-cALFA

    Plasmid

    #160499

    Purpose

    For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    human P2X3 (P2RX3 Human, Synthetic)
    

    Tags

    HRV3C protease cleavage site-ALFA tag-twin Strep …

    Expression

    Mammalian

    Mutation

    ΔN5ΔC33-T13P/S15V/V16I

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-ARP8

    Plasmid

    #15361

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    actin-related protein 8 (ACTR8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. Diacylglycerol Kinase Zeta

    Plasmid

    #85454

    Purpose

    expression of Diacylglycerol Kinase Zeta

    Depositor

    Stephen Gee

    Article

    Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

    Insert

    Diacylglycerol Kinase Zeta (DGKZ Human)
    

    Tags

    3x HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSR357.24

    Plasmid

    #125109

    Purpose

    Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid

    #53225

    Purpose

    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

    Depositor

    Jesse Rinehart

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Phosphoprotein synthesis

    Tags

    HIS and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli, both codons for Ser21…

    Promoter

    pLtetO

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Promoter

    H1/TO

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. lentiMutB3GALT5catdead-blast

    Plasmid

    #220869

    Purpose

    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    encodes D156A and D243A mutations; also, nucleoti…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBAD-SPW-RHH

    Plasmid

    #190630

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW-RHH

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW-RHH
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUBC_HA_PP7-mVenus

    Plasmid

    #111268

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mVenus
    

    Use

    Synthetic Biology

    Tags

    HA, SV40 NLS, and mVenus

    Expression

    Mammalian

    Mutation

    PP7: C68S, S69G [delta]70-75 (non-aggregating)

    Promoter

    hUBC

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. AAV_Actb HMEJ donor

    Plasmid

    #97318

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. CYFIP2.mCherry

    Plasmid

    #122052

    Purpose

    Expresses human CYFIP2 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid

    #200910

    Purpose

    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    3XHA_den1FnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Glutamic Acid to Arginine at 1369 position

    Promoter

    CBH

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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