-
Plasmid#195108PurposeTargeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system.DepositorInsertAID
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F2
Plasmid#184167PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F1
Plasmid#184166PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-07
Plasmid#124786PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP2
Plasmid#26282DepositorInsertMSP2
UseTags6-HisExpressionBacterialMutationsynthetic gene of dimer of deletion mutant (1-43)…PromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z34
Plasmid#120062PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-26
Plasmid#120005PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Adnp
Plasmid#74340PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Adnp. Not for gene expression.DepositorInsertactivity-dependent neuroprotective protein (Adnp Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z21
Plasmid#120064PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4DBD-3xP3-RFP
Plasmid#127564PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4DBD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P12
Plasmid#120023PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarletDepositorInsertmScarlet
UsePcr templateTagsmScarletExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA
Plasmid#133339Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-p65-3xP3-RFP
Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z20
Plasmid#120063PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntagDepositorInsert10xSuntag
UsePcr templateTags10xSuntagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
UseTagsExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172Available sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z23
Plasmid#120066PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-QF2-3xP3-RFP
Plasmid#127559PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.DepositorInsertQF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P06
Plasmid#120017PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-23
Plasmid#120002PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z28
Plasmid#120071PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P09
Plasmid#120020PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenusDepositorInsertmVenus
UsePcr templateTagsmVenusExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMBA323
Plasmid#214751PurposeTo create delta(glmS,nadE) working strains and maintaining their viabilityDepositorInsertBBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP
Plasmid#205008PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.DepositorInsertmEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mCherry
Plasmid#205002PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
XW08
Plasmid#65832PurposePunc-4-QF-SL2-mcherry-3'UTR-unc-54DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationPromoterPunc-4Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMVS-V1
Plasmid#183094PurposeBasic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicusDepositorInsertspME2001
thermostable neomycin phosphotransferase gene
UseBacterial propagation and conjugativeTagsExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P31
Plasmid#120042PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5DepositorInsertV5
UsePcr templateTagsV5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-phoA
Plasmid#207991PurposeCRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertphoA
UseCRISPRTagsExpressionMutationPromoterAspergillus flavus U6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-12
Plasmid#119991PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mScarletDepositorInsertmScarlet
UsePcr templateTagsmScarletExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GI-ZFP2
Plasmid#42215PurposeExpresses GI-ZFP2 for light-inducible activation of gene expressionDepositorInsertGIGANTEA-FLAG-ZFP2 (GI Mustard Weed, Synthetic)
UseTagsFLAGExpressionMutationPromoterCMVAvailable sinceJan. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pgWIZ-SGCAgl
Plasmid#131456PurposeEncodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imagingDepositorInsertSGCA (SGCA Human)
UseTags3xFLAGExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P02
Plasmid#120013PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFPDepositorInsertTagBFP
UsePcr templateTagsTagBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pMaCTag-Z09
Plasmid#120052PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenusDepositorInsertmVenus
UsePcr templateTagsmVenusExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z33
Plasmid#120050PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA.008
Plasmid#22841DepositorInsertA DNA (with MCS)
UseCotton vigs vectorTagsExpressionBacterialMutationCoat Protein (AR1 gene) of Cotton Leaf Crumple Vi…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-15
Plasmid#119994PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z17
Plasmid#120060PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P14
Plasmid#120025PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5DepositorInsertmNeptune2.5
UsePcr templateTagsmNeptune2.5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z15
Plasmid#120058PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-22
Plasmid#120001PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-11
Plasmid#119990PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA
Plasmid#133342Purposefor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P15
Plasmid#120026PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only