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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,361 results for: actin

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Showing: 1801 - 1850 of 3361 results

  5. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-mCh-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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  9. DHFR-mNeon-GluA1

    Plasmid
    #173009
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-mNeon-GluA1
    Tags
    DHFR and mNeon
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173009-plasmid-map-sequence-id-339113

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  28. Myopathy Loop Mutant Nuclear Myosin I YFP

    Plasmid
    #60243
    Purpose
    Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity
    Depositor
    Primal de Lanerolle
    Article
    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.
    Insert
    Nuclear Myosin 1 (Myo1c Mouse)
    Tags
    EYFP and NLS
    Expression
    Mammalian
    Mutation
    Deleted I339, I340, G343, E344, E345,L346
    Promoter
    CMV
    Available Since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pExpreS2-1-C3-PA

    Plasmid
    #175446
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A and BiP Secretion Peptide
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175446-plasmid-map-sequence-id-358138

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  31. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Article
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    129531-plasmid-map-sequence-id-255632

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  46. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  47. pExpreS2-1-CR-PA

    Plasmid
    #175445
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    BiP Secretion Peptide and Thrombin-Protein A
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175445-plasmid-map-sequence-id-358137

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Showing: 1801 - 1850 of 3361 results