We narrowed to 9,088 results for: ef1;
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Plasmid#164791PurposeExpress miR-144 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralExpressionMammalianPromoterEF1Alpha and U6Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only
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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
Plasmid#164800PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1DepositorInsertsERH CRISPRi gRNA1
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromoterEF1Alpha and U6Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2
Plasmid#164801PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2DepositorInsertsERH CRISPRi gRNA2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromoterEF1Alpha and U6Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75232PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA
Plasmid#37089PurposeExpresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationΔOB deleted 125 a.aPromoteref1-aAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA1-lenti
Plasmid#115662PurposeExpresses AcrVA1 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA1
UseLentiviralExpressionMammalianPromoterEF-1a promoterAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE
Plasmid#129704PurposeExpress HA-KASH for nuclei labeling and purificationDepositorInsertHA-VHH-KASH
UseAAV and Cre/LoxTagsHAExpressionMammalianPromoterEF1aAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75237PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOTTC710 - pAAV EF1a DIO Mem-AcGFP
Plasmid#75081PurposeAn AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.DepositorInsertMem-AcGFP
UseAAV and Cre/LoxTagsPalmitylation siteExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE-SOX17-P2A-Venus-rpEF1a-Zeo
Plasmid#169174PurposepiggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175DepositorInsertSOX17-P2A-Venus (SOX17 Human, Synthetic)
UsePiggybac transposonExpressionMammalianPromoterpTREtight (tet-inducible promoter)Available SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode
Plasmid#170254PurposeBarcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA
Plasmid#37088PurposeExpresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Archon1-KGC-GFP-ER2
Plasmid#115890PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianPromoterEF1a(1.1kb short version)Available SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast
Plasmid#71219PurposedCas9 fused to the human DNMT3B catalytically inactive domainDepositorInsertdSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
UseLentiviralTagsdSpCas9-DNMT3B(E697A)ExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-mouse SmyD1-T2A-copGFP
Plasmid#101157PurposeLentiviral expression of SmyD1 and copGFPDepositorAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)
Plasmid#100061PurposeAAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoterDepositorHas ServiceAAV9InsertChR1-VChR1 Chimera
UseAAVTagsmCherryExpressionMammalianMutationE122T and E162TPromoterEF1aAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVExpressionMammalianPromoterEF1α promoterAvailable SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin
Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)
Plasmid#139230PurposemMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)
Plasmid#159658PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRPromoterEf1aAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)
Plasmid#159657PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRPromoterEf1aAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1-fDIO-mNeon-WPRE-PolyA
Plasmid#121546PurposeSuper-bright fluorescence protein for tracking and visualizationDepositorInsertcre-dependent mNeon
UseAAV, Cre/Lox, and Synthetic BiologyAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30
Plasmid#163359PurposeLentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.DepositorAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75244PurposeCRISPR/Cas9 plasmid against human HeliosDepositorInsertsgRNA against human Helios (IKZF2 Human)
UseCRISPRAvailable SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP
Plasmid#113156PurposeAn AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporterDepositorInsertTwo gRNAs for rat Rosa26
UseAAV and CRISPRExpressionMammalianPromotermU6 and hU6Available SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL
Plasmid#63884PurposeAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.DepositorInsertER-localized GCaMP3(wt)
UseAAVPromoterEF1aAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75233PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PBG-WPRE-hGH
Plasmid#74287PurposepAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent mannerDepositorInsertPBG (chimeric rabies Glycoprotein)
UseAAVPromoterEf1aAvailable SinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-EGFP-miR-MCS (JDW 1478)
Plasmid#242580PurposeAn EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.DepositorInsertEGFP intron and miR-MCS (empty)
ExpressionMammalianPromoterhuman EF1aAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA
Plasmid#247646Purposemammalian expression of NLS-MARIO, a genetically-encoded fluorescent Mg2+ sensorDepositorInsertMARIO
TagsNLSExpressionMammalianAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Pgc-1alpha-EYFP
Plasmid#241793PurposeExpression vector for mouse Pgc-1alpha with EYFPDepositorAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT
Plasmid#247334PurposeExpresses histone H2B fused with mRFP1, together with monomeric EGFPDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-att-ef1a-MS2-RecT-dCas9-BSD
Plasmid#226108PurposeUsing ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selectionDepositorInsertBSD
UseCRISPRExpressionMammalianAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP
Plasmid#245377PurposeCre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.DepositorAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2Psub-Kv-WPRE
Plasmid#247323PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-2Psub-Kv
UseAAV and Cre/LoxTagsKvExpressionMammalianPromoterEF1aAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2Psub-WPRE
Plasmid#247224PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector under the control of the mammalian promoter (EF1a)DepositorInsertJEDI-2Psub
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo
Plasmid#239609PurposeBackbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistanceDepositorInsertfiller-trcr-H1/7SKhybrid-filler
UseCRISPR and LentiviralAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FRT-MCS-WPRE-SV40 - GCH15.1
Plasmid#241328PurposeAAV cloning vector for Flp dependent expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FRT-MCSrev-WPRE-SV40 - GCH18.1
Plasmid#241334PurposeAAV cloning vector for Flp dependent expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FLEX-Gephyrin.FR-GFP-p2A-mRuby
Plasmid#245310PurposeExpression of synaptic GFP (no transcriptional control) and cytosolic mRubyDepositorInsertsGephyrin.FR-GFP-p2A-mRuby
Gephyrin.FR-GFP-p2A-mRuby
UseAAVExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-EGFP-hs-miR-126 (JDW 1415)
Plasmid#242581PurposeAn EF1a driven expression vector containing an EGFP fused to the human microRNA 126.DepositorInsertEGFP with an intron containing hs-miR-126 (MIR126 Human)
ExpressionMammalianPromoterhuman EF1aAvailable SinceSept. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP11-22-122-EGFP-Hygro
Plasmid#238097PurposeExpression of structurally predicted WWE domain of PARP11 with EGFP on C terminalDepositorInsertPARP11 (PARP11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 22-112 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP12-298-462-Hygro
Plasmid#238098PurposeExpression of structurally predicted WWE domain of PARP12 with EGFP on C terminalDepositorInsertPARP12 (PARP12 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 298-462 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARPT-334-406-Hygro
Plasmid#238099PurposeExpression of structurally predicted WWE domain of PARPT with EGFP on C terminalDepositorInsertPARPT
UseLentiviralTagsEGFPExpressionMammalianMutationaa 334-406 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP14-1535-1601-Hygro
Plasmid#238100PurposeExpression of structurally predicted WWE domain of PARP14 with EGFP on C terminalDepositorInsertPARP14 (PARP14 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1535-1601 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-ZCCHV-594-684-Hygro
Plasmid#238102PurposeExpression of structurally predicted WWE domain of ZCCHV with EGFP on C terminalDepositorInsertZCCHV
UseLentiviralTagsEGFPExpressionMammalianMutationaa 594-684 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-RNF146-45-182-EGFP-Hygro
Plasmid#238091PurposeExpression of structurally predicted WWE domain of RNF146 with EGFP on C terminalDepositorInsertRNF146 (RNF146 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 45-182 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only