We narrowed to 17,810 results for: SHE

1. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Promoter

   hEF-1a

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FAT FAK biosensor

   Plasmid

   #78303

   Purpose

   FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

   Insert

   FAT FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLL3.7m-psMEK1tight

   Plasmid

   #89362

   Purpose

   A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psMEK1tight (MAP2K1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-3x-FLAG-Ubiquitin (JDW 1098)

   Plasmid

   #224513

   Purpose

   A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   3x-FLAG-Ubiquitin
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN34-BiNLS-hYAP1-1delta

   Plasmid

   #169471

   Purpose

   expresses optoYAP in mammalian cells

   Depositor

   Timothy Saunders

   Article

   Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

   Insert

   YAP1-1delta (YAP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.2_3xFLAG_V5_ eGFP_V5

   Plasmid

   #160111

   Purpose

   Expresses eGFP in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   3xFLAG, V5

   Expression

   Mammalian

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Tags

   HindIII site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Rep2

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

   Plasmid

   #222854

   Purpose

   Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRAV25

    Plasmid

    #237982

    Purpose

    Encodes TRAV25 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Article

    Gaglione et al bioRxiv 2025.04.24.649726

    Insert

    TRBC-P2A-TRAV25 (TRAV25 Human)
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218863

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX304_zeo_eGFP

    Plasmid

    #160095

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-mito-LAR-GECO1.2

    Plasmid

    #61245

    Purpose

    Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

    Depositor

    Robert Campbell

    Article

    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

    Insert

    LAR-GECO1.2
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1.2: N45I/A47R/E1…

    Promoter

    CMV

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. TK-GFP-Nluc-P2A-neo-TK

    Plasmid

    #134896

    Purpose

    Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Insert

    TK-GFP-Nluc-P2A-Neo-TK
    

    Use

    Cryptosporidium expression

    Promoter

    Cryptosporidium actin and enolase

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-mmCdh5pro (JDW 1239)

    Plasmid

    #224478

    Purpose

    Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    Cdh5 promoter
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHEN-CoV2-nanobody-F1

    Plasmid

    #194592

    Purpose

    Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    F1 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEN-CoV2-nanobody-E2

    Plasmid

    #194590

    Purpose

    Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    E2 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

    Plasmid

    #214881

    Purpose

    Lentiviral vector encoding RfxCas13d targeting B2M guide

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crB2M-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7m-psMEK1

    Plasmid

    #89361

    Purpose

    A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK1 (MAP2K1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRBV6-4

    Plasmid

    #237916

    Purpose

    Encodes TRBV6-4 allele and TRAC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Article

    Gaglione et al bioRxiv 2025.04.24.649726

    Insert

    TRBV6-4-TRAC (TRBV6-4 Human)
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. TRBV27

    Plasmid

    #237947

    Purpose

    Encodes TRBV27 allele and TRAC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Article

    Gaglione et al bioRxiv 2025.04.24.649726

    Insert

    TRBV27-TRAC (TRBV27 Human)
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TRAV19

    Plasmid

    #237976

    Purpose

    Encodes TRAV19 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Article

    Gaglione et al bioRxiv 2025.04.24.649726

    Insert

    TRBC-P2A-TRAV19 (TRAV19 Human)
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRAV12-2

    Plasmid

    #237968

    Purpose

    Encodes TRAV12-2 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Article

    Gaglione et al bioRxiv 2025.04.24.649726

    Insert

    TRBC-P2A-TRAV12-2 (TRAV12-2 Human)
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid

    #123363

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    EGFP-NLS
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRK5-FLAG-SLC38A9.1

    Plasmid

    #71855

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pHEN-CoV2-nanobody-C7

    Plasmid

    #194591

    Purpose

    Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    C7 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-FRT-TO-NOD1-EGFP

    Plasmid

    #131206

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD1 (NOD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST-FRT/T0-GFP-BRCA1

    Plasmid

    #71116

    Purpose

    mammalian expression of GFP-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PD-1-miSFIT-4x

    Plasmid

    #124678

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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