We narrowed to 13,271 results for: cDNA

1. 2xHP1bCD_W42A-IFP2.0C

   Plasmid

   #215751

   Purpose

   Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2xHP1bCD_W42A-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmyc-GFP-TNRC6A-(457-924)

   Plasmid

   #42006

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa457-924

   Promoter

   CMV

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-Golgin-84-TEV #709#243

   Plasmid

   #42108

   Depositor

   Ayano Satoh

   Article

   Koreishi et al Antibody Technology Journal. 2011;1:5-11

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   inserted ENLYFQG b/w 300 and 301

   Promoter

   CMV

   Available Since

   Feb. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

4. 5HT6-YC3.60

   Plasmid

   #48339

   Purpose

   FRET reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
   

   Use

   Tags

   YC3.60

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. BirA-Myc_N_MYC

   Plasmid

   #167715

   Purpose

   Expresses MYC in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

   Plasmid

   #169548

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcpAzpaRS_v2

   Plasmid

   #196483

   Purpose

   To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40(synthetase), Pol III tRNA promoter (tRNA), C…

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. POLArISact

   Plasmid

   #164971

   Purpose

   Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   POLArISact
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CoV2-N-P.1

   Plasmid

   #177959

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P80R,R203K,G204R

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SSTR2-DuET

    Plasmid

    #213377

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR2 (SSTR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TPI-PTC160

    Plasmid

    #130698

    Purpose

    Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC160 (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    Pcmv

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP-CD28TMD-NTEVp(H75E)

    Plasmid

    #137829

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75E)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PTPRA_deltaD1-SHC

    Plasmid

    #139615

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA_deltaD1 (PTPRA Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    D1 domain deletion

    Promoter

    CMV

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTNR1A-DuET

    Plasmid

    #213348

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1A (MTNR1A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. bArr2-ALFA

    Plasmid

    #201498

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr2-ALFA (ARRB2 Human)
    

    Use

    Tags

    ALFA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTracer_Cr-TRP1

    Plasmid

    #64881

    Purpose

    Expresses CrTRP1 in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

    Insert

    Transient receptor potential 1
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. miniABEMax V82G wt

    Plasmid

    #135362

    Purpose

    Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    miniABEMax nSpyCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dynamin-I-K44A-6His/Flag

    Plasmid

    #32696

    Depositor

    Robert Lefkowitz

    Article

    Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

    Insert

    Dynamin I (Dnm1 Rat)
    

    Use

    Tags

    Flag and His6

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

21. ALIX(1-702)-mNeonGreen

    Plasmid

    #191193

    Purpose

    Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (1-702) (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Deletion of ALIX C-terminal PRD.

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Diacylglycerol Kinase Zeta

    Plasmid

    #85454

    Purpose

    expression of Diacylglycerol Kinase Zeta

    Depositor

    Stephen Gee

    Article

    Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

    Insert

    Diacylglycerol Kinase Zeta (DGKZ Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-ARP8

    Plasmid

    #15361

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    actin-related protein 8 (ACTR8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Mfn1(K88T)-10xmyc

    Plasmid

    #26050

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    10xMyc

    Expression

    Mammalian

    Mutation

    K88T GTPase mutant

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

25. Flag-V0D1

    Plasmid

    #210348

    Purpose

    Expresses ATP6V0D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0D1 (ATP6V0D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203K

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. cpGFP-Adhiron_flex

    Plasmid

    #165044

    Purpose

    Flexible linker control for POLArISact expression in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    cpGFP-Adhiron (connected with a flexible linker)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. DCT.VV.Orf45

    Plasmid

    #12733

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf45
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

29. CaV1.3e[8a,11,31b,Δ32,42a] mut

    Plasmid

    #26576

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

30. SnFR-gamma2

    Plasmid

    #165495

    Purpose

    Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge