We narrowed to 21,881 results for: tes

1. p3E-Rac1-DN

   Plasmid

   #109582

   Purpose

   Multisite gateway vector for 3' tagging with dominant negative Rac1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rac1 (rac1a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   dominant negative

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. actc1b-mKate2-Rac1

   Plasmid

   #109529

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to Rac1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rac1 (rac1a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. actc1b-mKate2-Rac1-DN

   Plasmid

   #109530

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rac1 (rac1a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   DN

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2p2A--3mnx1:mTagBFP-NS-MCS

   Plasmid

   #78250

   Purpose

   Can be used to subclone in gene of interest into blue motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -mTagBFP-NS-MCS
   

   Use

   Zebrafish motor neuron expression

   Tags

   Expression

   Mutation

   Promoter

   -3mnx1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ML1Nmut_in_pEGFP-C1

   Plasmid

   #67796

   Purpose

   Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2(7Q)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-IRES-tdTomato-FlagAkt1W80A

   Plasmid

   #64833

   Purpose

   cDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drug

   Depositor

   Eva Gonzalez

   Article

   Kajno et al Biochem J. 2015 Apr 9.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Point mutation from Trp to Ala at position 80

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-MLC2(CA)-IRES-GFP

   Plasmid

   #133928

   Purpose

   Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EGFP and MLC2(CA) (MYL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MLC2 T18D, S19D

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-loxP-mTagBFP2-stop-pA-loxP

   Plasmid

   #75158

   Purpose

   Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   loxP-mTagBFP2-stop-pA-loxP
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A52V

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Expression

    Mutation

    Promoter

    hUbC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Biomolecular FeedBack (FB)

    Plasmid

    #109391

    Purpose

    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pJ23100mut-dCas9-T- phtpG1-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-p3E-HyPer7

    Plasmid

    #194289

    Purpose

    Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    Hyper7
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pTW072M

    Plasmid

    #115960

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl2 (nsP2 Q739L)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    nsP2 Q739L

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFlc245

    Plasmid

    #101021

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTet-O-OVOL1-T2A-PuroR

    Plasmid

    #162347

    Purpose

    Lentiviral expression of OVOL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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