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Showing: 761 - 780 of 4045 results

  1. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104990-plasmid-map-sequence-id-443800

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  2. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  3. DsRed2-Mito-7

    Plasmid
    #55838
    Purpose
    Localization: Mitochondria, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Use
    Tags
    DsRed2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55838-plasmid-map-sequence-id-244346

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  8. pAAV-CaMKIIa-EGFP

    Plasmid
    #50469
    Purpose
    EGFP under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50469-plasmid-map-sequence-id-171178

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  9. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  11. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  17. T444T

    Plasmid
    #113081
    Purpose
    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113081-plasmid-map-sequence-id-248277

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Showing: 761 - 780 of 4045 results