2,172 results
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mKate2-DrCavin1b in pT3TS-Dest
Plasmid#194295PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LACDepositorInsertcavin1b (cavin1b Zebrafish)
UseIn vitro transcription of mrnaAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…Available SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5E-3x125bp-mnx1
Plasmid#169043Purpose5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.DepositorAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGCaMP6s-P2A-H2AmCherry
Plasmid#170209PurposeDrives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleusDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
Plasmid#140893PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian celDepositorTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE), cha…Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -2
Plasmid#180009PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:mCherry
Plasmid#113881Purposep3E entry vector for Three Multisite GatewayDepositorAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
MutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)Available SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Acvr1ba-P2A-Acvr2ba
Plasmid#232640PurposeOptogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expressionDepositorInsertAcvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
Available SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCK033 p5E desma MCS
Plasmid#195948PurposeGateway p5E vectorDepositorInsertdesmin a regulatory elements (desma Zebrafish)
UseOtherAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ins:CFP-NTR
Plasmid#66593PurposeSpecific and conditional cell ablation of beta cells in the zebrafish pancreasDepositorInsertInsulin promotor driving CFP-nitroreductase
UseZebrafish expressionTagsCFP fused with N-terminus of nitroreductasePromoterZebrafish Insulin promoter (approximately 1.2kb)Available SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-zFGFR1a
Plasmid#232639PurposeZebrafish FGFR1a receptor kinase domain fused to VfLOVDepositorInsertzFGFR1a kinase domain + VfLOV domain
Available SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-zTRAP
Plasmid#42236DepositorInsertEGFP-Rpl10a (rpl10a Zebrafish)
TagsEGFPAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFRT-TODestGFP_RNAseH1
Plasmid#65784Purposeexpresses GFP-tagged RNAseH1 in mammalian cellsDepositorAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+Paxillin-mKate
Plasmid#105974Purposein vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesionsDepositorInsertPaxillin
TagsmKateExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mpeg:lamp1b-mCherry-stop; cmlc2:GFP
Plasmid#213739PurposeExpresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis markerDepositorInsertlamp1b-mCherry
UseZebrafishPromotermpeg1.1Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -2
Plasmid#180012PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-dusp6
Plasmid#82584Purpose5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cellsDepositorInsertFGF-responsive element of the dusp6 promoter
PromoterNoneAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-mitfa2.1
Plasmid#81234Purposezebrafish mitfa promoter entry vector for multisite Gateway cloningDepositorInsertmitfa promoter (mitfa Zebrafish)
UseMultisite gateway entry vectorPromotermitfa, 2.1kb including 5' UTRAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2
Plasmid#97145PurposeFor Gal4/UAS expression systemDepositorInsertDendra2
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP-IRES2-EGFP
Plasmid#80333PurposeExpresses Danio rerio VSP with separated EGFP in mammalian cellDepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only
