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We narrowed to 845 results for: trna

Showing: 121 - 160 of 845 results

  1. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  2. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  3. pGlu57

    Plasmid
    #211560
    Purpose
    Amber Suppressor tRNA for Glutamate
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAGlu
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211560-plasmid-map-sequence-id-438928

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  4. pGly57

    Plasmid
    #211561
    Purpose
    Amber Suppressor tRNA for Glycine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAGly
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211561-plasmid-map-sequence-id-438930

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  5. pHis57

    Plasmid
    #211562
    Purpose
    Amber Suppressor tRNA for Histidine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAHis
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211562-plasmid-map-sequence-id-438922

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  6. pLeu57

    Plasmid
    #211563
    Purpose
    Amber Suppressor tRNA for Leucine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALeu
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211563-plasmid-map-sequence-id-417239

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  7. pPhe57

    Plasmid
    #211568
    Purpose
    Amber Suppressor tRNA for Phenylalanine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAPhe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211568-plasmid-map-sequence-id-438920

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  8. pPro57

    Plasmid
    #211569
    Purpose
    Amber Suppressor tRNA for Proline
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAPro
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211569-plasmid-map-sequence-id-438933

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  9. pSer57

    Plasmid
    #211570
    Purpose
    Amber Suppressor tRNA for Serine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNASer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211570-plasmid-map-sequence-id-421061

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  10. pAla57

    Plasmid
    #211556
    Purpose
    Amber Suppressor tRNA for Alanine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAAla
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211556-plasmid-map-sequence-id-417300

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  11. pArg57

    Plasmid
    #211557
    Purpose
    Amber Suppressor tRNA for Arginine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAArg
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211557-plasmid-map-sequence-id-417160

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  12. pCys57

    Plasmid
    #211558
    Purpose
    Amber Suppressor tRNA for Cysteine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNACys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211558-plasmid-map-sequence-id-438923

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  13. pGln57

    Plasmid
    #211559
    Purpose
    Amber Suppressor tRNA for Glutamine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNAGln
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211559-plasmid-map-sequence-id-417453

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  14. pLys57-1

    Plasmid
    #211564
    Purpose
    Amber Suppressor tRNA for Lysine
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211564-plasmid-map-sequence-id-438925

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  15. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  16. pLys57-2

    Plasmid
    #211565
    Purpose
    Amber Suppressor tRNA for Lysine (Methionine-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211565-plasmid-map-sequence-id-421056

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  17. pLys57-3

    Plasmid
    #211566
    Purpose
    Amber Suppressor tRNA for Lysine (Isoleucine-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211566-plasmid-map-sequence-id-421057

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  18. pLys57-4

    Plasmid
    #211567
    Purpose
    Amber Suppressor tRNA for Lysine (Aspartate-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211567-plasmid-map-sequence-id-421059

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  19. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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  20. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  23. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

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  27. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

    Enlarge

  28. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  29. Lmir_GluTR_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228387
    Purpose
    Expresses N-term transit peptide from Lophophytum mirabile glutamyl-tRNA reductase (TRINITY_DN3595_c0_g2_i6); determination of L. mirabile glutamyl-tRNA Reductase target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. mirabile glutamyl-tRNA reductase (TRINITY_DN3595_c0_g2_i6)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228387-plasmid-map-sequence-id-461175

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  30. Lpyr_GluTR_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228390
    Purpose
    Expresses N-term transit peptide from Lophophytum pyramidale glutamyl-tRNA reductase (TRINITY_DN24865_c0_g2_i5); determination of L. pyramidale glutamyl-tRNA reductase target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. pyramidale glutamyl-tRNA reductase (TRINITY_DN24865_c0_g2_i5)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228390-plasmid-map-sequence-id-461829

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  31. Balfun_GluTR_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228384
    Purpose
    Expresses N-term transit peptide from Balanophora fungosa glutamyl-tRNA reductase (GWHDONK00038241); determination of B. fungosa glutamyl-tRNA Reductase target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from B. fungosa glutamyl-tRNA reductase (GWHDONK00038241)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228384-plasmid-map-sequence-id-461174

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  33. pAS

    Plasmid
    #140008
    Purpose
    Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    4xPylT (Mma) cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    U25C
    Promoter
    h7SK
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140008-plasmid-map-sequence-id-296092

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  36. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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  37. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

    Enlarge

  38. pDule-Tet3.0

    Plasmid
    #174080
    Purpose
    Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    Tet3.0 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L270G, Y271L, N311G, C313A
    Promoter
    Available since
    Jan. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    174080-plasmid-map-sequence-id-351340

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  40. pcDNA3.1-IARS2-K664A/K667A

    Plasmid
    #232339
    Purpose
    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative tRNA binding deficiency mutation K664A A…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232339-plasmid-map-sequence-id-474533

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Showing: 121 - 160 of 845 results