59,428 results

1. pGL3-noSV40-humanCOL2A1enhancer

   Plasmid

   #173961

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer region from COL2A1 gene
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-55:DMD-mEGFP

   Plasmid

   #159744

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_SPP1_WT_V5

   Plasmid

   #82981

   Purpose

   Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SPP1 (SPP1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX301-KANK1

   Plasmid

   #121985

   Purpose

   overexpress of KANK1

   Depositor

   GuangJun Zhang

   Article

   Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

   Insert

   KANK1 (KANK1 Human)
   

   Use

   Lentiviral

   Promoter

   CMV promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5VBD (USP09X)

   Plasmid

   #101260

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP09X (USP9X Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pVITRO-HPV38 L1L2

   Plasmid

   #52594

   Purpose

   Expresses HPV38 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV38 L1
   HPV38 L2
   

   Expression

   Mammalian

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

   Plasmid

   #52502

   Purpose

   expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   deletion of Fbox (deleted aa 71-116)

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-7 asyn S129A

   Plasmid

   #36048

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein S129A (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   S129A

   Promoter

   T7

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC2A12

   Plasmid

   #132067

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A12 (SLC2A12 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

    Plasmid

    #171963

    Purpose

    Donor plasmid for CLTX T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a-tm CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLuc-3'UTR

    Plasmid

    #42094

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 3UTR (ZEB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET SUMO NL-FKBP-Cas9

    Plasmid

    #162730

    Purpose

    Bacterial expression, protein expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO HIS HSPA6

    Plasmid

    #19540

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_RIMS2_WT

    Plasmid

    #81803

    Purpose

    Gateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RIMS2 (RIMS2 Human)
    

    Use

    Gateway entry vector

    Mutation

    S86F; Q295R

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-Rab8a-TN

    Plasmid

    #86080

    Purpose

    A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSN-A112C-GpA-G83I

    Plasmid

    #191239

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and Glycin…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSN-A112C-GpA

    Plasmid

    #191238

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ14566

    Plasmid

    #239272

    Purpose

    Expresses TRF1-PYL1 for recruiting RNA to the telomere via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    TRF1-BFP-PYL1 (TERF1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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