We narrowed to 18,729 results for: MUT

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pRK5-HA-YFP-Raptor K840R

Plasmid

#204418

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 840 to Arginine (K840R)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K1097R

Plasmid

#204417

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 1097 to Arginine (K1097R)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMM289

Plasmid

#46037

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN E84A/K577M (WRN Human)

Use

Tre, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

E84A/K577M exonuclease and helicase-dead mutant; …

Promoter

CMV2

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMM287

Plasmid

#46035

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN K577M (WRN Human)

Use

Teton, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

K577M helicase-dead mutant; S2G introduced during…

Promoter

CMV2

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pegRNA_LMNA_CR-2-5

Plasmid

#199275

Purpose

pegRNA used to correct LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824TtoC pegRNA

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMM288

Plasmid

#46036

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN E84A (WRN Human)

Use

Tre, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

E84A exonuclease-dead mutant; S2G introduced duri…

Promoter

CMV2

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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raACR-mCerulean3-pAAV2-hSyn

Plasmid

#204966

Purpose

Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-I997A

Plasmid

#111832

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A) (VCL Chicken)

Use

Lentiviral

Mutation

changed vinculin isoleucine 997 to alanine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_TAL1

Plasmid

#167728

Purpose

Expresses TAL1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TAL1 (TAL1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE_tau-LacZ::tTA-H100Y

Plasmid

#34927

Depositor

Mark Mayford

Article

Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

Inserts

tau (MAPT Bovine)
tTA

Use

Mouse Targeting

Tags

LacZ

Expression

Mammalian

Mutation

Made doxycycline insensitive by introduction of a…

Promoter

tetO

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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YF-Rac1(CA)

Plasmid

#20150

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(CA) (RAC1 Human)

Expression

Mammalian

Mutation

constitutively active mutation

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Plasmid

#135454

Purpose

Bacterial expression of TEVΔ protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability. Truncated form…

Promoter

tac promoter

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAID 1.1 N-T2A-Bsr

Plasmid

#105985

Purpose

To make an auxin inducible degron mutant

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

Insert

T2A-Bsr

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4(C744E)/2797

Plasmid

#11206

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

cysteine 744 mutated to glutamic acid

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
hRIP1 HA GFP K45A

Plasmid

#41389

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

Kinase Domain Mutant (K45A)

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
TALED_Right-ND1-1397N-UGI

Plasmid

#183899

Purpose

Expresses TALED_Right-ND1-1397N-UGI in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

DDCBE for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

Plasmid

#182009

Purpose

CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

IgKchL_HA_STf_myc_PDGFRtmb

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Nck

Plasmid

#186133

Purpose

For expression of recombinant Nck in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NCK1 (NCK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acid residues 1-377, with mutations: C139S,…

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x5

Plasmid

#58775

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA_sh_resistant

Plasmid

#105006

Purpose

Mammalian Expression of PDZD8, sh resistant

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Mutation

The sequence recognized by shPDZD8 (TRCN000043105…

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p35S-A3A-PBE

Plasmid

#119773

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP PR-D25A

Plasmid

#101334

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Mutation

Protease D25A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B(T116C)

Plasmid

#157808

Purpose

Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B(T116C)

Expression

Bacterial

Mutation

T116C

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

MARCKS(1-40) (MARCKS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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