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173,051 results

Showing: 1201 - 1230 of 173051 results

  2. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  4. pCV020-tetQ

    Plasmid
    #232826
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - tetracycline
    Depositor
    Athanasios Typas
    Insert
    tetQ
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232826-plasmid-map-sequence-id-462958

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  7. pLPC-JAG1-mVenus

    Plasmid
    #171170
    Purpose
    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    JAG1 (JAG1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    171170-plasmid-map-sequence-id-339658

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  10. pcDNA3.1-HA

    Plasmid
    #128034
    Purpose
    Mammalian expression vector with N terminal HA tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128034-plasmid-map-sequence-id-422971

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  14. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-492918

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  18. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  21. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid
    #212961
    Purpose
    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_backbone
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212961-plasmid-map-sequence-id-422482

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  25. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  26. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  28. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  29. TFORF1974

    Plasmid
    #143988
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX3 (TBX3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143988-plasmid-map-sequence-id-385902

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Showing: 1201 - 1230 of 173051 results