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We narrowed to 14,123 results for: cas9 genes

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Showing: 2001 - 2050 of 14123 results

  1. dCas9-NLS-gs10-cDHFR174

    Plasmid
    #188256
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cDHFR174 (DHFR Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188256-plasmid-map-sequence-id-374594

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  4. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid
    #104412
    Purpose
    transient expression of dCas9-dDNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    dDNMT3a
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    C706S
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104412-plasmid-map-sequence-id-204224

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  6. PX406 Pasteurella multocida Cas9

    Plasmid
    #68703
    Purpose
    Pasteurella multocida Cas9
    Depositor
    Feng Zhang
    Insert
    PmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

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  8. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  9. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  11. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62133-plasmid-map-sequence-id-101417

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  12. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107305-plasmid-map-sequence-id-204646

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  13. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

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  18. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

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  25. PX390 Flavobacterium columnare Cas9

    Plasmid
    #68327
    Purpose
    Flavobacterium columnare Cas9
    Depositor
    Feng Zhang
    Insert
    FcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68327-plasmid-map-sequence-id-119543

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  30. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  32. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid
    #129444
    Purpose
    expression of FnCas9 and DsRed
    Depositor
    Oskar Laur
    Insert
    FnCas9 IRES-Dsred
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129444-plasmid-map-sequence-id-256492

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  43. p-dCas9-SET7-H297G-Hygro

    Plasmid
    #104415
    Purpose
    transient expression of dCas9-dSET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    dSET7
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H297G
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104415-plasmid-map-sequence-id-203185

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  44. PX395 Roseburia intestinalis Cas9

    Plasmid
    #68332
    Purpose
    Roseburia intestinalis Cas9
    Depositor
    Feng Zhang
    Insert
    RiCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68332-plasmid-map-sequence-id-119548

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  45. pMuLE ENTR SV40-hCas9 L5-L2

    Plasmid
    #62134
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62134-plasmid-map-sequence-id-101418

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  46. Cas9 ROSA sgRNA mCherry

    Plasmid
    #155280
    Purpose
    Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155280-plasmid-map-sequence-id-309987

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  50. pMuLE ENTR CMV-hCas9 R4-R3

    Plasmid
    #62132
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62132-plasmid-map-sequence-id-101416

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Showing: 2001 - 2050 of 14123 results