161,805 results

1. pJethlpAmScarletCm

   Plasmid

   #206818

   Purpose

   Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

   Depositor

   Virginie Libante

   Article

   Mignolet et al bioRxiv 2025.05.10.653270

   Insert

   hlpA
   

   Use

   Synthetic Biology

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJethlpAYFPCm

   Plasmid

   #206826

   Purpose

   Template for hlpA-YFP amplification associated with chloramphenicol resistance gene

   Depositor

   Virginie Libante

   Article

   Mignolet et al bioRxiv 2025.05.10.653270

   Insert

   hlpA
   

   Use

   Synthetic Biology

   Tags

   YFP

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJethlpAmNeonGreenCm

   Plasmid

   #206814

   Purpose

   Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

   Depositor

   Virginie Libante

   Article

   Mignolet et al bioRxiv 2025.05.10.653270

   Insert

   hlpA
   

   Use

   Synthetic Biology

   Tags

   mNeonGreen

   Expression

   Mutation

   3insG

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJethlpAmNeonGreenKm

   Plasmid

   #206816

   Purpose

   Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

   Depositor

   Virginie Libante

   Article

   Mignolet et al bioRxiv 2025.05.10.653270

   Insert

   hlpA
   

   Use

   Synthetic Biology

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ec dnaN-pHK/P

   Plasmid

   #237239

   Purpose

   over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

   Depositor

   Michael O'Donnell

   Insert

   dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
   

   Use

   Tags

   his-10+PKA kinase motif+PreScission Protease clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT37

   Plasmid

   #223409

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT40

   Plasmid

   #223412

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT10

   Plasmid

   #223382

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT07

   Plasmid

   #223379

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

    Plasmid

    #235150

    Purpose

    Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    HaloTag linked to SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

    Plasmid

    #231372

    Purpose

    Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

    Depositor

    David Bartel

    Article

    Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

    Insert

    Human Argonaute2 (AGO2 Synthetic, Human)
    

    Use

    Tags

    3xFLAG-SUMO_Eu1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMC00035 (aka pMG0784)

    Plasmid

    #210768

    Purpose

    Lentiviral vector to express human ADRM1

    Depositor

    Raj Chari

    Article

    Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

    Insert

    ADRM1 (ADRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBWB514

    Plasmid

    #209326

    Purpose

    p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

    Depositor

    Adam Arkin

    Article

    Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCambia-dLB-kanR-GFP

    Plasmid

    #223177

    Purpose

    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

    Depositor

    Andreas Stuermer

    Type

    Empty backbone

    Use

    Ti plasmid for agrobacterium

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBES1-LacOx50-blast

    Plasmid

    #210221

    Purpose

    Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEL2319

    Plasmid

    #195697

    Purpose

    Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    Foldon-MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

    Plasmid

    #178991

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBTetD-SNAP-Cep27

    Plasmid

    #136809

    Purpose

    Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep27 (HAUS2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. rs214-minor

    Plasmid

    #113380

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2149943 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWN_U6-B2M-miniGag

    Plasmid

    #228957

    Purpose

    miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge