165,775 results

1. AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163476

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Promoter

   Beta Globin minimal promoter

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Expression

   Insect

   Promoter

   nanos

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Tobias Meyer, Sabrina Spencer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PRS_8-GCaMP8m

   Plasmid

   #210400

   Purpose

   AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

   Depositor

   Simon Wiegert

   Article

   Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

   Insert

   GCaMP8m
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   PRSx8

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-Trx

   Plasmid

   #112577

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-TrxA and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-6xHis-AviTag-ALFAtag-eGFP

   Plasmid

   #195854

   Purpose

   Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   6xHis-AviTag-ALFAtag-eGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExpreS2-1-GFP

   Plasmid

   #175363

   Purpose

   ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Insert

   sgGFP
   

   Expression

   Insect

   Mutation

   F64L, S65C, and I167T

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. w+attB

   Plasmid

   #30326

   Depositor

   Jeff Sekelsky

   Insert

   phiC31 attB, hsp70::w
   

   Use

   Drosophila transgenesis

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

    Plasmid

    #73164

    Purpose

    multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

    Depositor

    Bruce Edgar

    Article

    Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

    Insert

    mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
    

    Tags

    mRFP1, GFP

    Expression

    Insect

    Mutation

    Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3s-ND5-Right-G1397-N

    Plasmid

    #169213

    Purpose

    Express DdCBE for ND5 Point Mutation

    Depositor

    Jin-Soo Kim

    Article

    Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

    Insert

    DdCBE for ND5 mutation
    

    Expression

    Mammalian

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3s-ND5-Left-G1397-C

    Plasmid

    #169212

    Purpose

    Express DdCBE for ND5 Point Mutation

    Depositor

    Jin-Soo Kim

    Article

    Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

    Insert

    DdCBE for ND5 mutation
    

    Expression

    Mammalian

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid

    #202528

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. circRNA-synIRES-R25-NanoLuc

    Plasmid

    #188116

    Purpose

    Template plasmid to transcribe circular RNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE7-P2A-GFP

    Plasmid

    #222996

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLCAR-CD19-BBz

    Plasmid

    #135992

    Purpose

    Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-TurboID

    Plasmid

    #124646

    Purpose

    Expresses Flag-TurboID fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-evoCAST-Cas6

    Plasmid

    #234725

    Purpose

    Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseCas6
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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