-
Plasmid#224558Purposeexpresses Keima fused to PEX11B in mammalian cellsDepositorInsertPEX11B (PEX11B Human)
UseRetroviralTagsKeimaExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IP APEX-PEX26
Plasmid#224562Purposeexpresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cellsDepositorInsertPEX26 (PEX26 Human)
UseRetroviralTagsAPEXExpressionMammalianMutationPEX26 peroxisomal targeting sequencePromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_mCherry
Plasmid#221470PurposeGateway cloning of mCherry overexpressionDepositorInsertmCherry
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA179
Plasmid#215939PurposeFragmid fragment: (guide cassette) short sequence with no known functionDepositorHas ServiceCloning Grade DNAInsertFiller
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM
Plasmid#206206PurposeConstitutive expression of BsmAIM methyltransferaseDepositorInsertBsamAIM
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-HA-BioID2-SAPAP3
Plasmid#176857PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in neuronsDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-ADCK5-V5-miniTurbo_IDG-K
Plasmid#189909PurposeGateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertADCK5 (ADCK5 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-COQ8A-V5-miniTurbo_IDG-K
Plasmid#189885PurposeGateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCOQ8A (COQ8A Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mPpp3cc-FLAG-6XHIS
Plasmid#179160PurposeExpression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
UseTags6XHIS and FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab32b
Plasmid#140885PurposeExpression of zebrafish GFP-tagged Rab32b in mammalian cells.DepositorInsertRab32b (rab32b Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64-GFP-ePDZ-CAAX
Plasmid#135603PurposeExpression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ-CAAX
UseIn vitro transcription (mrna synthesis)TagsCAAX, EGFP, and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-membrane-mCherry-LOV
Plasmid#135599PurposeExpression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertmembrane-mCherry-LOV
UseIn vitro transcription (mrna synthesis)TagsLOV and mCherryExpressionMutationPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19a-3p
Plasmid#103323PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19a-3p target (MIR19A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DGKQ gRNA (BRDN0001162275)
Plasmid#77377Purpose3rd generation lentiviral gRNA plasmid targeting human DGKQDepositorInsertDGKQ (DGKQ Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K3 gRNA (BRDN0001148097)
Plasmid#76016Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K3DepositorInsertMAP3K3 (MAP3K3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
UseTagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26b)ExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits