165,528 results

1. pET-28a(+)-T7-OmpA-MNaseB-6xHis

   Plasmid

   #214809

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease B
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLF1-C_CSNK2A2:1-350

   Plasmid

   #211081

   Purpose

   Mammalian expression of full-length human CSNK2A2. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CSNK2A2:M1-R350 (CSNK2A2 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

   Plasmid

   #213728

   Purpose

   Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SlugCas9-P2A-puro

   Plasmid

   #214353

   Purpose

   Expresses SlugCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 and puromycin resistance genes
   

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pfa6a mScarlet-FKBP::KANMX

   Plasmid

   #185905

   Purpose

   Plasmid for cloning mScarlet fused to FKBP into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-FKBP
   

   Tags

   mScarlet fused to FKBP

   Expression

   Yeast

   Promoter

   SP6

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47811-pAtAct2::Cas9-nls::tSlATPase

   Plasmid

   #160221

   Purpose

   Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtACT2::Cas9-nls::tSlATPase
   

   Use

   Synthetic Biology; Moclo toolkit level 1 acceptor…

   Expression

   Plant

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2C-EGFP

   Plasmid

   #69071

   Purpose

   Expresses EGFP under MERVL_LTR

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nsp13-mCherry

   Plasmid

   #165136

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp13 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE60 R130A wgMDH

   Plasmid

   #204266

   Purpose

   Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. iRFP-H2A

    Plasmid

    #158691

    Purpose

    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    iRFP670
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3.1_V5-TWIST1

    Plasmid

    #215030

    Purpose

    Mammalian expression of human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-Tol1.zf1

    Plasmid

    #61388

    Purpose

    mRNA synthesis of tol1 transposase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    tol1.zf1
    

    Use

    Zebrafish expression

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pExchange_tdk_BT1311p-tetR-1

    Plasmid

    #90325

    Purpose

    Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    TetR
    

    Expression

    Bacterial

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-GFP-Kir2.1

    Plasmid

    #107184

    Purpose

    mammalian expression of Kir2.1

    Depositor

    Bikash Pattnaik

    Insert

    KCNJ2 (KCNJ2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJJS001

    Plasmid

    #188324

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::GFP::Linker
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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