159,530 results

1. iRFP-PH-PLCdelta1

   Plasmid

   #66841

   Purpose

   expression of near-infrared PI(4,5)P2 biosensor

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   PH-PLCdelta1
   

   Use

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LT3GEPIR-CCND1shRNA1

   Plasmid

   #220577

   Purpose

   To inducibly knockdown CCND1 expression

   Depositor

   Sean Lee

   Article

   Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

   Insert

   CCND1 shRNA1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. shCD44-2 pRRL

   Plasmid

   #19123

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL Hygro-NMYC TRF2

   Plasmid

   #16348

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF2 (TERF2 Human)
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   aa2-500

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pNCS-mClover3

   Plasmid

   #74236

   Purpose

   Constitutive expression of mClover3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Has Service

   Cloning Grade DNA

   Insert

   mClover3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZS157 CRISPEY RT/Cas9

   Plasmid

   #114454

   Purpose

   Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Insert

   SpCas9, Ec86-RT
   

   Use

   CRISPR

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   S. cerevisiae codon optimized

   Promoter

   GAL1-GAL10

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. JME4599

   Plasmid

   #129660

   Purpose

   NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

   Plasmid

   #140005

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry PP1 gamma

   Plasmid

   #45220

   Depositor

   Michael Lampson, Iain Cheeseman

   Article

   Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

   Insert

   mCherry PP1 gamma
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-EF1a-Con/Fon-GCaMP6M

    Plasmid

    #137119

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-EF1a_GFP

    Plasmid

    #221481

    Purpose

    GFP lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcS-p88-C1C2

    Plasmid

    #178424

    Purpose

    p88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domain

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    p88
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179012

    Purpose

    Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

    Plasmid

    #179008

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSG5-HA-ERR3 S45A

    Plasmid

    #37849

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    Estrogen-related receptor gamma (Esrrg Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Serine 45 to Alanine (AGC to GCC)

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL-p5X

    Plasmid

    #150814

    Purpose

    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Type

    Empty backbone

    Use

    Tags

    malE A313V

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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