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Showing: 2101 - 2150 of 2924 results

  11. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

    Plasmid
    #124068
    Purpose
    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Article
    He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1
    Insert
    eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Insect
    Mutation
    Promoter
    bhsp68
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124068-plasmid-map-sequence-id-240659

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  13. pSR13

    Plasmid
    #69155
    Purpose
    read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II
    Depositor
    Sander van den Heuvel
    Article
    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10
    Inserts
    mCherry
    swsn-1 (swsn-1 Nematode)
    eGFP
    Use
    Cre/Lox; Mossci
    Tags
    Expression
    Worm
    Mutation
    codon-optimzed index 1.0, codon-optimzed index 1.…
    Promoter
    rps-27 and swsn-1
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69155-plasmid-map-sequence-id-121941

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  19. pRosa26-GT

    Plasmid
    #40025
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    beta-globin intron
    Neo
    tdT-3Myc
    diphteria toxin A
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    40025-plasmid-map-sequence-id-210933

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  20. pRosa26-TG

    Plasmid
    #40026
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    T (ATG)
    beta-globin intron
    Neo
    GFP
    diphteria toxin A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides 1-274 and insertion of FRT, …
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    40026-plasmid-map-sequence-id-210931

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  24. pNMSB96

    Plasmid
    #199316
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, C. elegans codon optimized …
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199316-plasmid-map-sequence-id-404765

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  25. pNMSB104

    Plasmid
    #199314
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, C. elegans codon optimized …
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199314-plasmid-map-sequence-id-404775

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  26. pNMSB84

    Plasmid
    #199309
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199309-plasmid-map-sequence-id-404774

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  27. pNMSB99

    Plasmid
    #199308
    Purpose
    MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    myo-3
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199308-plasmid-map-sequence-id-404762

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  39. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164265-plasmid-map-sequence-id-330127

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  43. pAAV-nEF-Con/Fon-ChRmine-oScarlet

    Plasmid
    #137159
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Fon-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Tags
    Expression
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137159-plasmid-map-sequence-id-272775

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  47. pSicoR-Ef1a-mCh-Puro

    Plasmid
    #31845
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA; Ef1alpha for mCherry-puromycin
    Available since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31845-plasmid-map-sequence-id-194345

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  48. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

    Plasmid
    #166970
    Purpose
    Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.
    Depositor
    Matthew Banghart
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
    Use
    AAV and Cre/Lox
    Tags
    FLAG, T2A, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166970-plasmid-map-sequence-id-346867

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  49. LiOn-CMV∞Cre

    Plasmid
    #154018
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    Cre-FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154018-plasmid-map-sequence-id-308116

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Showing: 2101 - 2150 of 2924 results