Addgene

We narrowed to 13,557 results for: TIM

Showing: 2101 - 2150 of 13557 results

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pCIS.MTRIA-PGE

Plasmid

#184617

Purpose

For expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP657-SpHis5

Plasmid

#44846

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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Cox8x2-CeNL_pcDNA3

Plasmid

#89513

Purpose

Cyan color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-Cdc42

Use

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)

Plasmid

#163065

Purpose

Entry vector for: Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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1455_pDEST_miniTol2_R4-R2_MCS

Plasmid

#171794

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Cox8x2-ReNL_pcDNA3

Plasmid

#89533

Purpose

Red color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Article

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pEB2-Katushka

Plasmid

#104008

Purpose

Plasmid encoding Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pOz-humBirA-GFP

Plasmid

#133283

Purpose

Expression of BirA-GFP fusions in mammalian cells

Depositor

Arman Kulyyassov

Article

Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

Insert

eGFP

Use

Retroviral

Tags

Humanized wild type biotin ligase BirA

Expression

Mammalian

Mutation

Promoter

MoMuLV

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-Kan

Plasmid

#44957

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pMD18-T-pyrF

Plasmid

#174388

Purpose

modified pMD18-T containing pyrF and its native promoter, Ampr

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF

Use

Template for cloning fragments for chromosomal in…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mTagBFP2

Plasmid

#191746

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mTagBFP2

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Cox8x2-YeNL_pcDNA3

Plasmid

#89517

Purpose

Yellow color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoTagRFP657-Kan

Plasmid

#44955

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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VPS13D^Halo

Plasmid

#174108

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Use

Tags

Halo

Expression

Mammalian

Mutation

With an internal Halo tag in aminoacid position 1…

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-SpHis5

Plasmid

#44842

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Cox8x2-OeNL_pcDNA3

Plasmid

#89526

Purpose

Orange color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-beta2 TKIT donor

Plasmid

#169436

Purpose

Donor DNA for TKIT knockin of SEP to Gabrb2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_son-1_vsfGFP9

Plasmid

#191747

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and vsfGFP-9

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CIp10 - YemVenus (OPT High)

Plasmid

#163122

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

YemVenus OPT High

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.717

Plasmid

#108517

Purpose

PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTet*-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-moxVenus

Plasmid

#103993

Purpose

Plasmid encoding moxVenus

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxVenus

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-5HT

Plasmid

#184597

Purpose

For expression of a fluorescent sensor for serotonin in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-5HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.EF1a.FLEX.MTRIA-OT

Plasmid

#184595

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Tags

Expression

Mutation

Promoter

Human elongation factor-1 alpha promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomEos2-Kan

Plasmid

#44944

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.712

Plasmid

#108513

Purpose

PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCymRC-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomKeima-Kan

Plasmid

#44902

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.716

Plasmid

#108518

Purpose

PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBAD-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-HA-mTmod3-WT

Plasmid

#70752

Purpose

N-terminal-HA-tagged mouse Tropomodulin3, WT

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pCS-lpp0.2-K64A

Plasmid

#201789

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Use

Tags

Expression

Bacterial

Mutation

K64A

Promoter

Available since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCS-lpp0.2-H38A

Plasmid

#201788

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Use

Tags

Expression

Bacterial

Mutation

H38A

Promoter

Available since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CeNL-Actin_pcDNA3

Plasmid

#89512

Purpose

Cyan color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RT3REVIR

Plasmid

#111168

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Retroviral

Tags

dsRed and mVenus

Expression

Mutation

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mClover3

Plasmid

#103994

Purpose

Plasmid encoding mClover3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mClover3

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:SEP-GluA3 TKIT donor

Plasmid

#169425

Purpose

Donor DNA for TKIT knockin of SEP to GluA3 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoLSS-mKate2-Kan

Plasmid

#44943

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.714

Plasmid

#108515

Purpose

PVanCC-YFP Reporter. Van sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PVanCC-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LBJJ382

Plasmid

#117518

Purpose

Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX roGFP2-Prx2

Plasmid

#169230

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomMet26

Plasmid

#107448

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met26 (met26 Fission Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomMet16

Plasmid

#107452

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met16 (met16 Fission Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-pBAD

Plasmid

#54539

Purpose

Localization: Protein Expression Vector , Excitation: 555, Emission: 584

Depositor

Dorus Gadella, Michael Davidson, Dmitriy Chudakov

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Use

Tags

6xHis and mTagRFP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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