163,479 results

1. pwtCas9-bacteria

   Plasmid

   #44250

   Purpose

   aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   wild-type Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Promoter

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Neo DEST (705-1)

   Plasmid

   #17392

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV4 p50

   Plasmid

   #21965

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NF-kB p50 subunit (NFKB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-527

   Plasmid

   #11518

   Purpose

   Gateway destination vector for bacterial expression; His tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-HA-PD-L1

   Plasmid

   #121492

   Purpose

   Expression of human PD-L1 WT in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-EGFP-P2A-iCre (JDW 921)

   Plasmid

   #224519

   Purpose

   A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EGFP-P2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK18msB

   Plasmid

   #177839

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

   Depositor

   Christopher Johnson

   Article

   Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mCardinal

    Plasmid

    #51311

    Purpose

    Expresses far-red fluorescent protein mCardinal in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEmerald-Nesprin3-C-18

    Plasmid

    #54203

    Purpose

    Localization: Nuclear Membrane , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5B-HA-Smad2

    Plasmid

    #11734

    Depositor

    Jeff Wrana

    Article

    Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV2/11

    Plasmid

    #240486

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

    Depositor

    Addgene Research Program, Meghan Rego

    Insert

    AAV2 Rep and AAV11 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge