166,034 results

1. pC0041-RanCas13b crRNA backbone

   Plasmid

   #103852

   Purpose

   For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-RanCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-MST1

   Plasmid

   #1965

   Depositor

   Joseph Avruch

   Article

   Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

   Insert

   MST1 (Stk4 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. EIF2AK2 gRNA (BRDN0001148577)

   Plasmid

   #75638

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. beta2-spectrin-HA

   Plasmid

   #31070

   Depositor

   Vann Bennett

   Insert

   beta2-spectrin (SPTBN1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. EIF2AK3 gRNA (BRDN0001145848)

   Plasmid

   #77166

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK3 (EIF2AK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScarlessHD-DsRed

   Plasmid

   #64703

   Purpose

   Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Kate O'Connor-Giles

   Insert

   piggyBac-3xP3-DsRed
   

   Promoter

   3xP3

   Available Since

   Dec. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. gGBEv6.3-SpCas9

   Plasmid

   #202629

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpCas9(D10A)-MPGv6.3
   

   Expression

   Mammalian

   Mutation

   MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

   Promoter

   hU6, EF1a, CBh

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-14-3-3 eta

   Plasmid

   #116887

   Purpose

   Expresses HA-tagged human 14-3-3 eta in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Article

   Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

   Insert

   14-3-3 eta (YWHAH Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cyto-mTurq2-Apollo-NADP+

   Plasmid

   #193301

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Tags

   mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-endo-Nb80

    Plasmid

    #205518

    Purpose

    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb80
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-14-3-3 beta

    Plasmid

    #13270

    Depositor

    Michael Yaffe

    Insert

    14-3-3 beta (YWHAB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-14-3-3 epsilon

    Plasmid

    #116886

    Purpose

    Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    14-3-3 epsilon (YWHAE Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTEC15

    Plasmid

    #30174

    Depositor

    Lalita Ramakrishnan

    Article

    Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    Wasabi

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCaRNA1&2

    Plasmid

    #86087

    Purpose

    Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA1, RNA2
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-GFP1-10

    Plasmid

    #78591

    Purpose

    Express mCherry-GFP1-10 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    GFP 1-10
    

    Tags

    mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFPC1-human APPL1

    Plasmid

    #22198

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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