Addgene

We narrowed to 2,378 results for: gcg

Showing: 1351 - 1380 of 2378 results

Results per page:

Sort results by:

AURKB A4.4 gRNA

Plasmid

#90547

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKB (Guide Designation A4.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AURKB A3.4 gRNA

Plasmid

#90546

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKB (Guide Designation A3.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AURKB A3.1 gRNA

Plasmid

#90544

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKB (Guide Designation A3.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TGIF2 B9.4 gRNA

Plasmid

#90912

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGIF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TGIF2 (Guide Designation B9.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TGIF2 B10.4 gRNA

Plasmid

#90913

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGIF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TGIF2 (Guide Designation B10.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FZR1 D9.1 gRNA

Plasmid

#90692

Purpose

3rd generation lentiviral gRNA plasmid targeting human FZR1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FZR1 (Guide Designation D9.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FZR1 D10.1 gRNA

Plasmid

#90693

Purpose

3rd generation lentiviral gRNA plasmid targeting human FZR1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FZR1 (Guide Designation D10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MCM7 E4.2 gRNA

Plasmid

#90765

Purpose

3rd generation lentiviral gRNA plasmid targeting human MCM7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MCM7 (Guide Designation E4.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HJURP E4.1 gRNA

Plasmid

#90711

Purpose

3rd generation lentiviral gRNA plasmid targeting human HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

HJURP (Guide Designation E4.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
NDC80 F11.1 gRNA

Plasmid

#90786

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F11.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BUB3 A10.1 gRNA

Plasmid

#90557

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB3 (Guide Designation A10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MAD2L1BP F1.1 gRNA

Plasmid

#90744

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F1.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

Plasmid

#238167

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-miR30-shJAG1 #1

Plasmid

#171196

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #1 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #1

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pk335-CARGO-3mer-30kb-DSF

Plasmid

#227486

Purpose

3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 30kb Downstream Fgf5

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

Plasmid

#234883

Purpose

non-targeting CRISPRi control

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
shRNA_RNF170_1

Plasmid

#215206

Purpose

Supression of shRNF170_1 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

RNF170 shRNA 1 (RNF170 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL5.0_shRNAHsCavin1_EGFP

Plasmid

#229688

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Insert

shRNA for hCavin1 (CAVIN1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220117

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220120

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY2259 hNOLC1 atgRNA Paired Guide 1

Plasmid

#220989

Purpose

hNOLC1 atgRNA

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoA gRNA #1

Plasmid

#198718

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoC gRNA #1

Plasmid

#198724

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoC gRNA #3

Plasmid

#198726

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-nucGFP11-3xPax7gRNA

Plasmid

#224570

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Expression

Mutation

Codon optimized

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-3xGFP11-mem-3xPax7gRNA

Plasmid

#224571

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Expression

Mutation

Codon optimized

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-U6-E690_guide-CBh-hSpCas9

Plasmid

#188545

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge