We narrowed to 39,691 results for: Eras

1. AAV hSyn hPGK1-mRuby

   Plasmid

   #220911

   Purpose

   Neuronal specific expression of the fusion construct PGK1-mRuby

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-mRuby (PGK1 Human)
   

   Use

   AAV

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV2/9-CW3SL-mCherry-DrTrkB

   Plasmid

   #184002

   Purpose

   AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkB (NTRK2 Human)
   

   Use

   AAV

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLEX_305-N-dTAG_OGT

   Plasmid

   #155196

   Purpose

   N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   lacking N-terminal Met

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 145-pGL3ctrl-3' UTR

   Plasmid

   #28175

   Depositor

   Michael Kastan

   Article

   Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

   Insert

   Luciferase flanked by tp53 UTRs (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Note that insert length is based on sum of the 2 …

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pMiR-RECK-3'UTR

   Plasmid

   #53614

   Purpose

   Luciferase reporter assay for microRNA binding on the RECK 3'UTR

   Depositor

   Edward Chan

   Article

   Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

   Insert

   RECK-3'UTR (RECK Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. plenti hSyn PGK1-HALO

   Plasmid

   #220910

   Purpose

   Neuronal specific expression of the fusion construct PGK1-HALO

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-HALO (PGK1 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS416 Gal M337V YFP

   Plasmid

   #27449

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Methionine 337 mutated to Valine (M337V)

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-4T-1-β4 hinge-ear

   Plasmid

   #196692

   Purpose

   Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Psicheck2 COLIA1 full length 3'UTR

   Plasmid

   #26993

   Depositor

   Joan Massague

   Article

   Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

   Insert

   COL1A1 Full length 3' UTR (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLenti PGK OGT (Neo)

    Plasmid

    #154289

    Purpose

    Lentiviral vector encoding full length wildtype human OGT

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TOPO tTR-KRAB-rtTA3-Luc

    Plasmid

    #68449

    Purpose

    TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    

    Use

    Luciferase; Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYLL204

    Plasmid

    #53455

    Purpose

    Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

    Depositor

    Lisa Lyu, Leroy Liu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y814F

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mutation

    Promoter

    Gal4 response element minP and PGK

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS416 Gal G294A YFP

    Plasmid

    #27448

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pAAV_12xTEAD_luc2

    Plasmid

    #173004

    Purpose

    firefly luciferase reporter vector with 12x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    12xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. N-BLa 1.1

    Plasmid

    #88994

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCold TDP43 G294A

    Plasmid

    #27452

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. C-BLa 1.1

    Plasmid

    #88997

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    C-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMiR-E6AP-3'UTR

    Plasmid

    #53694

    Purpose

    Luciferase reporter assay for microRNA binding on E6AP 3'UTR

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    UBE3A 3'UTR (UBE3A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pRS416 Gal TDP43 M337V

    Plasmid

    #27460

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pRS416 Gal TDP43 Q331K

    Plasmid

    #27461

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pVENUS-N1-FNTA

    Plasmid

    #86859

    Purpose

    To express FNTA with a Venus tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    FNTA (FNTA Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    The sequence of the insert is isoform 2, which di…

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS416 Gal TDP43 G294A

    Plasmid

    #27459

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

24. Psicheck2 MERTK full length 3'UTR

    Plasmid

    #26992

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    MERTK Full length 3' UTR (MERTK Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

25. 908E

    Plasmid

    #160238

    Purpose

    TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Ttg Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 908G

    Plasmid

    #160239

    Purpose

    PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Pip Operator (3x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 908J

    Plasmid

    #160242

    Purpose

    CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Cym Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Psicheck2 Sox4 mut 3'UTR oligo

    Plasmid

    #26988

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 mut UTR oligo (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    bp 449-509 of SOX4 3'UTR; mut @ bp 483

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pJK597

    Plasmid

    #71121

    Purpose

    Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-SNAP-CD86tm-eGFP-FKBP(f36v)

    Plasmid

    #200280

    Purpose

    Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    SNAP-tag-CD86tm-eGFP-FKBP(f36v)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-eGFP-CD86tm-HALOtag-FKBP(f36v)

    Plasmid

    #200281

    Purpose

    Membrane protein fusion with extracellular GFP and intracellular FKBP(f36v) dimerization domain and HALOtag

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    eGFP-CD86tm-HaloTag-FKBP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET30-10His-FKBP(f36v)-SNAP

    Plasmid

    #200282

    Purpose

    For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    FKBP-SNAPtag
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV_8xTEAD_luc2

    Plasmid

    #173005

    Purpose

    firefly luciferase reporter vector with 8x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    8xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pminiTol2-4kb wnt3 promoter-Fzd1mApple

    Plasmid

    #203795

    Purpose

    Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesis

    Depositor

    Thorsten Wohland

    Article

    Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

    Insert

    Fzd1 (fzd1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mApple

    Expression

    Mutation

    Promoter

    4kb Wnt3 promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGBT9-GGA2-GAE

    Plasmid

    #199175

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA2-GAE domain (GGA2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Contains Lys 545 Gln substitution

    Promoter

    ADH1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGBT9-GGA3-GAE

    Plasmid

    #199176

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA3-GAE domain (GGA3 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGBT9-GGA1-GAE

    Plasmid

    #199174

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA1-GAE domain (GGA1 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGAD424-Rabex-5

    Plasmid

    #197043

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 908I

    Plasmid

    #160241

    Purpose

    VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Van Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGL3-PIM2mut3

    Plasmid

    #53742

    Purpose

    promoter region of PIM2 lacking the 2 forkhead binding sites

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    PIM2 (PIM2 Human)
    

    Use

    Luciferase; Reporter vector

    Tags

    Expression

    Mutation

    deletion of 2 forkhead binding sites (bp# 395-497…

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only