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MSCVpic2neo-hTERT-p16 shRNA

Plasmid

#164920

Purpose

Expresses hTERT cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

human telomerase reverse transcriptase (TERT Human)
CDKN2A (CDKN2A Human)

Use

Retroviral

Tags

HA tag

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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Bcl11b MSCV IRES mCherry

Plasmid

#189808

Purpose

Retroviral expression of Bcl11b

Depositor

Ellen Rothenberg

Insert

Bcl11b (Bcl11b Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-3xNLS-mScarlet

Plasmid

#191098

Purpose

To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-mScarlet

Use

AAV and Synthetic Biology

Tags

Three repeats of the nuclear localizzation seque…

Expression

Mammalian

Mutation

Optimized to human codon usage

Promoter

CMV enhancer and CAG

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-BirA-P2A-mCherry

Plasmid

#74056

Purpose

retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

BirA biotin ligase

Use

Retroviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

MSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

Plasmid

#229782

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

3x mScarlet-I

Use

Synthetic Biology

Tags

ITPKA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CREB3L1

Plasmid

#214767

Purpose

Mammalian expression of human CREB3L1

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

CREB3L1 (CREB3L1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Blast

Plasmid

#169221

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Blast

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-NLS-CBRed-d2-hygro

Plasmid

#139196

Purpose

non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBRed

Use

Luciferase and Retroviral

Tags

d2 PEST domain and nuclear localization signal (N…

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-mCherry

Plasmid

#220353

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p6614 MSCV-IP N-HAonly PXMP3

Plasmid

#35012

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PXMP3 (PEX2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pDRF1 mScarlet3-T2A-mTurquoise2

Plasmid

#219848

Purpose

equimolar expression of mScarlet3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Insert

mScarlet3-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Luciferase-IRES-NrasG12D

Plasmid

#60834

Purpose

Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

NrasG12D (Nras Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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p7274 MSCV-P C-FlagHA 16 ∆DLYC

Plasmid

#163304

Purpose

Expresses HPV16 E7 ∆DLYC

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 ∆DLYC

Use

Retroviral

Tags

FlagHA

Expression

Mutation

∆DLYC

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-EcMscL-TEV-mEGFP

Plasmid

#165097

Purpose

Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

Depositor

Neha Kamat

Article

Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

Insert

E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)

Use

Synthetic Biology

Tags

monomeric Enhanced Green Fluorescent Protein

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING HAQ

Plasmid

#102600

Purpose

Express STING HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagMLL2-pL-ENL(5613)

Plasmid

#20874

Depositor

Robert Slany

Article

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL2 (Kmt2d, MLLT1 Mouse, Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV-Flag-cMyc T58A

Plasmid

#20075

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only
piggybac-Syn-mScn8a-3xHA

Plasmid

#182555

Purpose

Expresses Nav1.6 tagged with 3xHA

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Insert

Scn8a (Scn8a Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Syn

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Ccdc134-IRES-BFP

Plasmid

#117266

Purpose

Retroviral overexpression of Ccdc134

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Ccdc134 (Ccdc134 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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