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We narrowed to 22,937 results for: Tes

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Showing: 2301 - 2350 of 22937 results

  6. CUP1-∆ssCPY*-mNeonGreen in pRS315

    Plasmid
    #221160
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221160-plasmid-map-sequence-id-450956

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  10. pLSLR

    Plasmid
    #51500
    Purpose
    Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Plant t-dna plasmid
    Available Since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51500-plasmid-map-sequence-id-78614

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  11. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  12. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Promoter
    virion-sense LIR and 2x35S
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51494-plasmid-map-sequence-id-78590

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  19. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

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  23. pFSD-SIBR003

    Plasmid
    #250973
    Purpose
    FSD expressing SIBR-Cas system with intron 3, contains restriction sites to introduce HR and spacer sequence
    Depositor
    Peter Sarin
    Article
    Gregorova et al bioRxiv 2026.03.05.709803
    Insert
    SIBR003 system (SIBR Intron 3 FnCas12a)
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    March 25, 2026
    Availability
    Academic Institutions and Nonprofits only
    250973-plasmid-map-sequence-id-500754

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  24. pFSD-SIBR004

    Plasmid
    #250974
    Purpose
    FSD expressing SIBR-Cas system with intron 4, contains restriction sites to introduce HR and spacer sequence
    Depositor
    Peter Sarin
    Article
    Gregorova et al bioRxiv 2026.03.05.709803
    Insert
    SIBR004 system (SIBR Intron 4 FnCas12a)
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    March 25, 2026
    Availability
    Academic Institutions and Nonprofits only
    250974-plasmid-map-sequence-id-500755

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  26. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake, Chris MacDonald
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

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  40. pT7-pelB

    Plasmid
    #157740
    Purpose
    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence and possible His6-tag (adds …
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157740-plasmid-map-sequence-id-334002

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  48. pREPK219H

    Plasmid
    #51492
    Purpose
    pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Insert
    Rep/RepA
    Use
    Plant expression t-dna
    Mutation
    changed lysine 219 to histidine
    Promoter
    2x35S
    Available Since
    May 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    51492-plasmid-map-sequence-id-78586

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  49. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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Showing: 2301 - 2350 of 22937 results