163,364 results

1. VinTS-tCRMod-GGSGGS9

   Plasmid

   #111765

   Purpose

   Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX304-GW-DCK*-IRES-GFP

   Plasmid

   #176291

   Purpose

   Gateway vector for use in generating POI-DCK* fusion reporter

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Insert

   Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S74E R104M D133A

   Promoter

   CMV

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMCP-Luc (MCP-1 promoter in pGL3-basic)

   Plasmid

   #40324

   Depositor

   Alexander Dent

   Article

   Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

   Insert

   MCP-1 promoter (Ccl2 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   contains MCP-1 promoter region through the nucleo…

   Promoter

   MCP-1

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE puro human IRS-1

   Plasmid

   #11025

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-EGFP-Cdc42(Q61L)

   Plasmid

   #12600

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (Q61L) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZS2-123

   Plasmid

   #26598

   Depositor

   Michael Elowitz

   Article

   Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

   Insert

   Synthetic
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDB179-DuraPETase

   Plasmid

   #218699

   Purpose

   Encodes codon optimized DuraPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DuraPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEmerald-Annexin-12

   Plasmid

   #53991

   Purpose

   Localization: Plasma Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Annexin (ANXA4 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFH-14c

    Plasmid

    #39541

    Depositor

    Katharina Strub

    Article

    Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

    Insert

    SRP14 (SRP14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-MKLP1

    Plasmid

    #70145

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSCV-FLAG-hHelios-IRES-GFP

    Plasmid

    #74047

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Helios (IKZF2) (IKZF2 )
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    encodes shorter version of human Helios

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-Rab11a-7

    Plasmid

    #56444

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. TFORF1181

    Plasmid

    #143475

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX21 (TBX21 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SIRT5 Flag

    Plasmid

    #13816

    Purpose

    Mammalian expression of human SIRT5 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT5 (SIRT5 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUCP20T-eyfp

    Plasmid

    #78466

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    eYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDestTol2pA2-U6:gRNA

    Plasmid

    #63157

    Purpose

    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRXIP-GFP-PI4K2A

    Plasmid

    #221760

    Purpose

    Stable expression of GFP-PI4K2A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge