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We narrowed to 2,753 results for: PGK

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Showing: 81 - 100 of 2753 results

  2. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Publication
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83890-plasmid-map-sequence-id-240902

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  6. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Publication
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Promoter
    human PGK1
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68375-plasmid-map-sequence-id-251912

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  7. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid
    #67977
    Purpose
    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers
    Depositor
    Kosuke Yusa
    Publication
    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d
    Insert
    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    Use
    Lentiviral
    Mutation
    Deleted BbsI site within WPRE
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67977-plasmid-map-sequence-id-118694

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  8. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42337
    Purpose
    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42337-plasmid-map-sequence-id-57166

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  16. GG-EBNA-OSK2M2L1-PP

    Plasmid
    #102902
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OSK2M2L1-gRNAs-PGK-puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102902-plasmid-map-sequence-id-198388

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Showing: 81 - 100 of 2753 results