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NG-ABEmax

Plasmid

#124163

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TadA-TadA(evo)-Cas9-NG

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Publication

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pHR_SFFV

Plasmid

#79121

Purpose

Lentiviral vector for constitutive expression of genes (SFFV promoter)

Depositor

Wendell Lim

Publication

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Publication

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Mutation

H134R

Promoter

CAG

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Publication

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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Flag-cIAP2/pRK5

Plasmid

#27973

Depositor

Xiaolu Yang

Publication

Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CIAP2 (BIRC3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Mutation

See depositor comments below.

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV-TetO-hNGN2-eGFP-Puro

Plasmid

#79823

Purpose

Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Publication

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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Lenti-multi-CRISPR

Plasmid

#85402

Purpose

Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

Depositor

Qin Yan

Publication

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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T7opt in pCAGGS

Plasmid

#65974

Purpose

Human codon-optimized T7 polymerase

Depositor

Benhur Lee

Publication

Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

Insert

T7-opt

Expression

Mammalian

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLM-mCitrine-Sox2

Plasmid

#23242

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Publication

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Publication

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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