We narrowed to 7,853 results for: 11
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Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only
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pEGFP-Merlin S518D
Plasmid#84295PurposeExpression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation siteDepositorAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-syn-fRCaMP2
Plasmid#125246PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRCaMP2
ExpressionMammalianMutationD396APromotersynapsinAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
TagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
shLuc pRRL
Plasmid#19125DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hUBP43
Plasmid#12455DepositorInsertUBP43 (USP18 Human)
TagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-R115A/R119A
Plasmid#80696PurposeExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-R115A/R119A
UseAAVExpressionMammalianMutationArg115 and Arg119 both mutated to AlaPromoterCBAAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC168
Plasmid#216350PurposexhNup35-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
FL-BCAN-lentiviral-GFP
Plasmid#29704DepositorInsertBrevican (BCAN Human)
UseLentiviralAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
ExpressionBacterialAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSC115
Plasmid#216356PurposexNup93-Nb2t Nanobody productionDepositorInsertxNup93-Nb2t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-H29A
Plasmid#80602PurposeExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-H29A
UseAAVExpressionMammalianMutationHis29 mutated to AlaPromoterCBAAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-HypaCas9-mRuby2
Plasmid#183872PurposepX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.DepositorTypeEmpty backboneUseCRISPRTagsHypaCas9-P2A-mRuby2-T2A-PuroExpressionMammalianPromoterCbhAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
Available SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0096 RESCUE truncation (Cas13 delta 892-1095)
Plasmid#130663PurposeCMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUEDepositorInsertdRanCas13b(delta 892-1095)-ADAR2dd
ExpressionMammalianAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDG04163
Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
ExpressionBacterialPromoterT7Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ910
Plasmid#44481DepositorInsertMinimal Mos1, NeoR, MCS
UseMultiple cloning site vectorAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
ER50-SpCas9-ER50
Plasmid#85448PurposeExpresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cellsDepositorInsertER50-SpCas9-ER50
UseAAVTagsdestabilized domain of estrogen receptor ligand b…ExpressionMammalianPromoterCbhAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSC234
Plasmid#216357PurposexhNup93-Nb3t (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb3t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn1beta AS4(-)
Plasmid#58267PurposeExpression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
TagsN-myristoylationExpressionInsectPromoterGAL4-responsive UAS hsp70Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryPromoterDHFR 5' UTRAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
ExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuroATT
Plasmid#47539PurposeRetroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)DepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ909
Plasmid#44480DepositorInsertMinimal Mos1, unc-119, MCS
UseMultiple cloning site vectorAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Friend MLV Env YFP
Plasmid#1820DepositorAvailable SinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
TagsN-myristoylationExpressionInsectAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
Plasmid#74101Purposevector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015DepositorInsertsGreen Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
Available SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBJ1 neo-hu FceRI gamma
Plasmid#16540DepositorInsertFCER1G (FCER1G Human)
ExpressionMammalianAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.col…ExpressionMammalianPromoterEF1aAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSC226
Plasmid#216375PurposexhNup358-Nb2t (+3Cys) Nanobody productionDepositorInsertxhNup358-Nb2t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCtnnd2.1-GFP
Plasmid#209095PurposeGFP expressing shRNA targeting Ctnnd2 N-terminusDepositorInsertshCtnnd2.1
Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_3
Plasmid#60564PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_3
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101103PurposeEncodes dCas9(C)-VP64 with SAM components fused to VEGFR1DepositorInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pReporter_4
Plasmid#60565PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_4
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTP728
Plasmid#216374PurposexNup358-Nb1t (+3Cys) Nanobody productionDepositorInsertxNup358-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRosa26 R1-ccdB-R2 RexNeo PI-SceI
Plasmid#24418DepositorInsert
UseMouse TargetingAvailable SinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
TagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh20.1 GFP
Plasmid#209106PurposeGFP expressing scramble of shRNA targeting Cdh20DepositorInsertscrCdh20.1
Available SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ906
Plasmid#44472DepositorInsertMinimal Mos1, unc-119, [4-3] Gateway
UseGateway CloningAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
rs214-minor
Plasmid#113380Purposeevaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)DepositorInsertrs2149943 minor allele
UseLuciferaseAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS1479-HTP-KanMX6
Plasmid#154085PurposeFor C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.DepositorInserthis6-TEV-protein A-KanR
UsePcr-based tagging in yeastTagshis6-TEV-protein AAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP11 WT puroR
Plasmid#46747PurposeRetroviral vector that expresses Flag-tagged wild type human USP11DepositorInsertUSP11
UseRetroviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only