We narrowed to 26,202 results for: STI

1. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMM131

   Plasmid

   #127214

   Purpose

   BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, AtSTM, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-NfsB.zf1

   Plasmid

   #61387

   Purpose

   mRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   nfsB.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   E28G

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-CMV-PM

   Plasmid

   #197386

   Purpose

   To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSW610 - GR-LhG4_BD

   Plasmid

   #115992

   Purpose

   entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   GR-LhG4_BD
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAID1.2-EF1a-NGFP-mAID

   Plasmid

   #140607

   Purpose

   Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPy-CAG-Cre::ERT2-IRES-BSD

   Plasmid

   #48760

   Purpose

   CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   Cre::ERT2
   

   Use

   Ppy

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOPS0377

   Plasmid

   #115509

   Purpose

   Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Constitutive promoter pNeo pOGG001

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ210

   Plasmid

   #109329

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC_mClover3_BSDminus_F0

    Plasmid

    #184165

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMOD_C'5802

    Plasmid

    #176879

    Purpose

    High constitutive expression of Firefly Luciferase in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    AtUbi10:Luc+:RbcsE9
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAID1.2-EF1a-NmCherry-mAID

    Plasmid

    #140603

    Purpose

    Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pECFP-Rac1Q61L

    Plasmid

    #105292

    Purpose

    Constitutively active Rac 1

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAID5XY

    Plasmid

    #165051

    Purpose

    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV0620

    Plasmid

    #133506

    Purpose

    Expression of eGFP tagged PCNA to monitor DNA replication

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAID1.2-CMV-NmCherry-mAID

    Plasmid

    #140601

    Purpose

    Expression of OsTIR1 and mCherry-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSW614 - pREV

    Plasmid

    #115989

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pREV
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV0785

    Plasmid

    #133507

    Purpose

    Expression of mCherry tagged PCNA to monitor DNA replication

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSW618 - pAHP6

    Plasmid

    #115991

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pAHP6
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSW615 - pATHB-8

    Plasmid

    #115990

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pATHB-8
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    A1287T to abolish Eco31I site

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMC_mClover3_BSDminus_F2

    Plasmid

    #184167

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOPS0750

    Plasmid

    #115511

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAID1.2-CMV-CmCherry-mAID

    Plasmid

    #140602

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAV0782

    Plasmid

    #133514

    Purpose

    Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAV0751

    Plasmid

    #133485

    Purpose

    Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pECFP-Cdc42Q61L

    Plasmid

    #105294

    Purpose

    Constitutively active Cdc42

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-flag-mKdm4d(H189A)-polyA

    Plasmid

    #61554

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (catalytic mutant)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    changed Histidine 189 (of Kdm4d) to Alanine

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AICSDP-144:CTCF-mEGFP

    Plasmid

    #168799

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

    Depositor

    Allen Institute for Cell Science

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pAV0752

    Plasmid

    #133486

    Purpose

    Expression in fission yeast from promoter pnmt41 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAID1.2-CMV-CGFP-mAID

    Plasmid

    #140606

    Purpose

    Expression of OsTIR1 and mAID-GFP protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV0753

    Plasmid

    #133487

    Purpose

    Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAIDFA-CMV-NmScarlet-mAID

    Plasmid

    #140616

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWPXL-NP1

    Plasmid

    #72654

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    NP1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOPS0314

    Plasmid

    #115507

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSW609 - N001

    Plasmid

    #115993

    Purpose

    intermediate vector for GreenGate cloning method, contains modules from A-F

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Unspecified

    Tags

    HDEL and SP(ER)

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pconst #5-RFP

    Plasmid

    #81079

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23110

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AICSDP-079:DCP1A-mEGFP

    Plasmid

    #193922

    Purpose

    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

    Depositor

    Allen Institute for Cell Science

    Insert

    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAV0749

    Plasmid

    #133483

    Purpose

    Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCMV-Tag 3A/WT myc-M1

    Plasmid

    #87719

    Purpose

    Expresses WT myc-M1 spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M1 isoform

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAV0758

    Plasmid

    #133511

    Purpose

    Expression of 3GFP tagged RasAct probe to monitor active Ras1

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAV0783

    Plasmid

    #133515

    Purpose

    Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

    Plasmid

    #91932

    Purpose

    Bacterial expression of maize SUMO1a and SCE1b

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1b-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23107

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

    Plasmid

    #91934

    Purpose

    Bacterial expression of maize SUMO1a and SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. UAS::PTPRFa.1-DN-GFP

    Plasmid

    #37110

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)
    

    Use

    Tol2 gateway expression vector

    Tags

    EGFP

    Expression

    Mutation

    deleted amino acids 1326-1894

    Promoter

    UAS-E1b

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge