Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,219 results for: cas9 plasmid

Clear keywords
Showing: 2501 - 2550 of 7219 results

  2. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available Since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

    Enlarge

  4. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

    Enlarge

  6. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

    Enlarge

  18. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62201-plasmid-map-sequence-id-228092

    Enlarge

  19. ABE9-SpRY

    Plasmid
    #242976
    Purpose
    A-to-G base editor fused to PAM-flexible SpRY-Cas9 nickase.
    Depositor
    Marc Freichel
    Article
    Ong et al Sci Rep. 2026 Feb 20;16(1):7463. doi: 10
    Insert
    pCMV-TadA-8e (N108Q/L145T)-nSpRYCas9
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    6*His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242976-plasmid-map-sequence-id-486100

    Enlarge

  22. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

    Enlarge

  24. pYPQ166

    Plasmid
    #109328
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.
    Insert
    zCas9
    Use
    CRISPR; Gateway compatible zcas9 entry clone
    Tags
    3x FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    109328-plasmid-map-sequence-id-210215

    Enlarge

  25. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

    Enlarge

  26. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

    Enlarge

  30. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

    Enlarge

  33. pNR2F1.1.0-gDNA

    Plasmid
    #112484
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1
    Depositor
    Kevin White
    Insert
    NR2F1 (NR2F1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112484-plasmid-map-sequence-id-230521

    Enlarge

  34. pFOXA3.1.0-gDNA

    Plasmid
    #112417
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3
    Depositor
    Kevin White
    Insert
    FOXA3 (FOXA3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112417-plasmid-map-sequence-id-230296

    Enlarge

  35. pELF4.1.0-gDNA

    Plasmid
    #112467
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4
    Depositor
    Kevin White
    Insert
    ELF4 (ELF4 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112467-plasmid-map-sequence-id-230497

    Enlarge

  36. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Expression
    Yeast
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

    Enlarge

  37. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161815-plasmid-map-sequence-id-320654

    Enlarge

  38. pRDA_837

    Plasmid
    #245328
    Purpose
    Cas9 CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245328-plasmid-map-sequence-id-488040

    Enlarge

  39. pX335 Mouse 5' Srcap gRNA A

    Plasmid
    #127904
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127904-plasmid-map-sequence-id-249683

    Enlarge

  40. pX330 Mouse 3' Ubf gRNA

    Plasmid
    #127902
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Mouse 3' Ubf
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127902-plasmid-map-sequence-id-249774

    Enlarge

Showing: 2501 - 2550 of 7219 results