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Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex4
Plasmid#85534PurposeInducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporterDepositorInsertmu p53
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_muBim_Ex3
Plasmid#85533PurposeInducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporterDepositorInsertmu Bim
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCN DSB Repair Reporter (DRR)
Plasmid#98895PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.DepositorInsertNeomycin-T2A-ISce1-ISce1-eGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG17-mCherry-HOTag3
Plasmid#190024PurposeTransiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.DepositorInsertLaG17 CluMPS
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5Flag2-P2A-smAID-mClover
Plasmid#110657PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5Flag2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5HA2-bglpA
Plasmid#110655PurposeLentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5HA2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5HA2-P2A-smAID-mClover
Plasmid#110658PurposeLentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5HA2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA
Plasmid#110654PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5Flag2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA
Plasmid#110656PurposeLentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5cmyc2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCF738
Plasmid#122032PurposeEF1a-WNV-protease-GFPDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLB
Plasmid#11619PurposepLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsGFPExpressionMammalianMutationPromotermouse U6Available sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
FuPick-shRNA
Plasmid#31614DepositorInsertPICK1 shRNA (Pick1 Rat)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-AP1x6-GM55
Plasmid#217416PurposeLuciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoter6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LAAPGW
Plasmid#205687PurposeshRNAmir backbone for RNA interference, with GFP reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterAHAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LHAPGW
Plasmid#205678PurposeshRNAmir backbone for RNA interference, with GFP reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterEHAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
8522-M01-663
Plasmid#225660PurposeLentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)DepositorInsertdsCopGFP
UseLentiviralTagsExpressionMutationPromoterSORE6-mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LMAPGW
Plasmid#205651PurposeshRNAmir backbone for RNA interference, with GFP reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEAPGW
Plasmid#205669PurposeshRNAmir backbone for RNA interference, with GFP reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBAPGW
Plasmid#205660PurposeshRNAmir backbone for RNA interference, with GFP reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterCBHAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LCAPGW
Plasmid#205642PurposeshRNAmir backbone for RNA interference, with GFP reporterDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterhCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV mCherry-DDX1-V5
Plasmid#175162PurposeLentiviral expression of human DDX1-V5DepositorInsertDDX1 (DDX1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-ITDE-AP1x6-DUSP5
Plasmid#217419PurposeLuciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDeDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Aiolos ZF2 (AA146-168) in Cilantro 2
Plasmid#74456PurposeMonitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorInsertAiolos (IKZF3) AA130-189 (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Aiolos AA130-189 in Pea
Plasmid#74459PurposeMonitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)DepositorInsertAiolos (IKZF3) AA130-189 (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huMcl-1.1
Plasmid#85530PurposeInducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporterDepositorInserthu Mcl-1.1 (MCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG2-wPRE-pA
Plasmid#60489PurposeeGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)DepositorInsertIRES-eGFP; wPRE; BGH-pA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC155
Plasmid#50949PurposeAn LV packaging vector expressing eGFPDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPI
Plasmid#12254Purpose2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsEMCV IRES-EGFP cassetteExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-C(3+)-EiP
Plasmid#21313PurposeLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterEOS-C(3+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-S(4+)-EiP
Plasmid#21314PurposeLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterEOS-S(4+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-PGK-EiP
Plasmid#21312PurposeControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
FpG5
Plasmid#69443Purposelentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAsDepositorInsertsminimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
10279-M28-663
Plasmid#225662PurposeLentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholdingDepositorInsertdsCopGFP
UseLentiviralTagsExpressionMutationPromotermCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV mCherry-DDX21-V5
Plasmid#175163PurposeLentiviral expression of human DDX21-V5DepositorInsertDDX21 (DDX21 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-shRnd3
Plasmid#86437Purpose2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFPDepositorInsertshRNA targeting RND3 (RND3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cilantro 2
Plasmid#74450PurposeCan clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-mMegf10 CAR
Plasmid#113013Purposefor production of lentivirus encoding the CD19 CAR-Pmegf10DepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL059
Plasmid#177210PurposeBicistronic vector with GFP reporterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Scr shRNA
Plasmid#59299Purposenon-targeting shRNADepositorInsertScr shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMK004
Plasmid#34616PurposeViral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicatorDepositorInsertArch D95N
UseLentiviralTagsGFPExpressionMammalianMutationChanged Aspartic Acid 95 to AsparaginePromoterCaMKIIAvailable sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
RAB14 shRNA
Plasmid#61496Purposeto knockdown endogenous RAB14 expression levelsDepositorInsertRAB14 (RAB14 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Lmo4.2 shRNA
Plasmid#59293PurposeshRNA to mouse Lmo4DepositorInsertLmo4.2 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Lmo4.3 shRNA
Plasmid#59292PurposeshRNA to mouse Lmo4DepositorInsertLmo4.3 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -