We narrowed to 3,402 results for: aaas

Showing: 2081 - 2120 of 3402 results

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CROP_C9_Puro_RET

Plasmid

#183319

Purpose

All-in-One CRISPRko system with a guide RNA that targets RET gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

RET

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PIK3CD

Plasmid

#183316

Purpose

All-in-One CRISPRko system with a guide RNA that targets PIK3CD gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PIK3CD

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_JAK3

Plasmid

#183304

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FYN

Plasmid

#183295

Purpose

All-in-One CRISPRko system with a guide RNA that targets FYN gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FYN

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CDK4

Plasmid

#183275

Purpose

All-in-One CRISPRko system with a guide RNA that targets CDK4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CDK4

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BTK

Plasmid

#183274

Purpose

All-in-One CRISPRko system with a guide RNA that targets BTK gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BTK

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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p8116 LentiCRISPR v2 sgPTPN14-4

Plasmid

#163316

Purpose

Expresses Cas9 and a human PTPN14-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgPTPN14-4

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYMCR2_Gb_PMLTSS

Plasmid

#140285

Purpose

CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cas9

Expression

Mammalian

Promoter

CBH

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_2

Plasmid

#135747

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_1

Plasmid

#135746

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLY133

Plasmid

#130961

Purpose

Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh119

Plasmid

#106789

Purpose

expression of gRNA targeting GCNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT6

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

Plasmid

#114730

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M7

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MLSE-shRPA3.457

Plasmid

#105584

Purpose

retrovirally express RPA3 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

RPA3 shRNA #457

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MLSE-shTAF12.364

Plasmid

#105577

Purpose

retrovirally express TAF12 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #364

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shTAF12.364

Plasmid

#105559

Purpose

retrovirally express of TAF12 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup88-XbaI

Plasmid

#87328

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - C16orf80 sgRNA 1

Plasmid

#70651

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against C16orf80

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
lentiCRISPR - C3orf17 sgRNA 2

Plasmid

#70653

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against C3orf17

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 Lmo3.5 shRNA

Plasmid

#59295

Purpose

shRNA to mouse Lmo3

Depositor

Ulrike Heberlein

Article

Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

Insert

Lmo3.5 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
MXW-PGK-c-let-7-LP3-IRES-EGFP

Plasmid

#37642

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP3 - AATATTACCA=>AAATTTACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Article

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcw107

Plasmid

#62511

Purpose

Gateway Expression Vector

Depositor

John Doench, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHS2002

Plasmid

#239832

Purpose

Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB omegaRNA scaffold

Expression

Mammalian

Mutation

Replacement of A12-A57 with GAAA in omegaRNA scaf…

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[SNCA]

Plasmid

#75437

Purpose

knockdown alpha-synuclein in rat neurons

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

snca (Snca Rat)

Use

AAV and RNAi

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

Plasmid

#197991

Purpose

For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

scramble shRNA (control)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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phage UBC NLS-HA-2XMCP-tagRFPt

Plasmid

#64541

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, NLS, and tagRFP-T

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-shWT1_B

Plasmid

#217637

Purpose

Dox inducible shRNA targeting WT-1

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shWT1_B (WT1 Human)

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAH750

Plasmid

#91212

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFUChW Parkin shRNA Mul1 shRNA

Plasmid

#78523

Purpose

Lentiviral vector encoding two shRNAs and mCherry reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)

Use

Lentiviral and RNAi

Promoter

H1

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#72619

Purpose

Expresses two gRNAs targeting GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward GFP

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

Plasmid

#179003

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pQCascade

Plasmid

#140622

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 3,402 results for: aaas