We narrowed to 6,084 results for: nls

1. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

   Plasmid

   #85821

   Purpose

   iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   Zf Ubi::cMyc oDam_f_GFPoNLS
   

   Use

   Tol2 based vector

   Tags

   cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

   Expression

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-FUS ΔNLS 1-513

   Plasmid

   #44984

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS ΔNLS (1-513aa) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acids 1-513

   Promoter

   tac

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pT2-7xTcf-NLS-YNL

   Plasmid

   #65711

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL
   

   Use

   Luciferase; Tol2 system

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGEV_2_+nls-eGFP-HA-_+1_BK+

   Plasmid

   #49312

   Purpose

   Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-EGFP-HA
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

   Plasmid

   #67687

   Purpose

   UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3796-38

   Insert

   dusp6 (dusp6 Zebrafish)
   

   Use

   Tags

   6xmyc-NLS-tdTomato-2A

   Expression

   Mutation

   S159A, S196A

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PHO4NLS-GFP-PRA (109)

   Plasmid

   #24044

   Depositor

   Michael Rout

   Insert

   PHO4
   

   Use

   Tags

   EGFP and PrA

   Expression

   Yeast

   Mutation

   Only bp 421-588 of PHO4 (NLS)

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pPHR-EYFP-NLS-VP16

   Plasmid

   #103822

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-VP16 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAB2NLS-GFP-PRA (107)

   Plasmid

   #24043

   Depositor

   Michael Rout

   Insert

   NAB2
   

   Use

   Tags

   EGFP and PrA

   Expression

   Yeast

   Mutation

   Only bp 601-750 of NAB2 (NLS)

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pEBB-GFP-hBEN-deltaNLS3

   Plasmid

   #22158

   Depositor

   Ananda Roy

   Article

   Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

   Insert

   hBEN (GTF2IRD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 883-889

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pSS22-IRES-NLS-eGFP

    Plasmid

    #241837

    Purpose

    Part I of the Donor construct for UBE3A reporter

    Depositor

    Albert Keung

    Article

    Kelkar et al Front Cell Dev Biol. 2025; 13:1665693. h

    Insert

    eGFP
    

    Use

    Donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PiggyBac-TDP-43deltaNLS-V5-APEX

    Plasmid

    #229081

    Purpose

    Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone

    Depositor

    Eran Hornstein

    Insert

    TDP-43deltaNLS (TARDBP Human)
    

    Use

    Tags

    APEX, BFP, and V5

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, K95A, K97A and R98A for mutatio…

    Promoter

    TRE3G

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-GRP78-NLS-Mut

    Plasmid

    #235206

    Purpose

    Expresses GRP78 with mutated NLS in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutate the NLS sequence

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrcHisA-NLS-BFP-mEosEM

    Plasmid

    #218934

    Purpose

    Express the nuclear import substrate NLS-BFP-mEosEM.

    Depositor

    Siegfried Musser

    Article

    Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

    Insert

    BFP-mEosEM fusion protein
    

    Use

    Tags

    6xHis and SV40 nuclear localization sequence (NLS…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. UBQ10pro-nlsABACUS2-400n-NosT

    Plasmid

    #203728

    Purpose

    Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10pro 1500bp

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI5-CasRx-NLS-HA (pKW919)

    Plasmid

    #229047

    Purpose

    Mammalian expression of CasRx-NLS-HA

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    CasRx (RfxCas13d)
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

    Plasmid

    #231568

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    All-in-one LOOMINA construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-APEX2-SOPP3-3xNLS

    Plasmid

    #229074

    Purpose

    expresses APEX2-SOPP3 in the mammalian nucleus

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid

    #227084

    Purpose

    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

    Depositor

    Chris Hill

    Insert

    spt6-tsh2 (SPT6 Budding Yeast)
    

    Use

    Tags

    STREPii-FLAG and SV40 NLS 1

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-AGG-NLS-EGFP

    Plasmid

    #214602

    Purpose

    express EGFP ; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    EGFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNLS-VP16-NIR-Fb(718)

    Plasmid

    #220745

    Purpose

    NLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-Fb(718)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    NLS-VP16

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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