We narrowed to 3,985 results for: LYC
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Plasmid#145607PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
FoxFTVC+bpFOG_MRAS-G22V
Plasmid#107521PurposeConstitutive activation of FGF/MAPK pathway by M-Ras-G22V mutantDepositorInsertM-Ras protein
UseTagsExpressionMutationchanged Glycine 22 to ValinePromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#115428PurposeExpresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1G108C-GG
Plasmid#85092PurposeHost vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationchanged Glycine 108 to CysteinePromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH (GB0064)
Plasmid#68165PurposeProvides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid partDepositorInsertSlNH promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPUbc (GB0145)
Plasmid#68170PurposeProvides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid partDepositorInsertSlUbc promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK315
Plasmid#71141PurposeExpression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to glycine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2A11 (GB0143)
Plasmid#68189PurposeProvides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid partDepositorInsertSl2A11 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTE8 (GB0144)
Plasmid#68190PurposeProvides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid partDepositorInsertSlE8 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244
Plasmid#17859DepositorInsertHaemophilus influenzae heat shock loci U and V
UseTagsExpressionBacterialMutationcoding sequence for residues 108-243 of HslU (the…PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys
Plasmid#15602DepositorInsertSup35 NM (SUP35 Budding Yeast, Candida albicans)
UseTags7xHis and CysteineExpressionBacterialMutation4 G/A. Four glycines mutated to alanines. The pos…PromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMD2-VSVGmut
Plasmid#182229PurposeExpresses VSVGmut protein in mammalian cells for lentiviral pseudotypingDepositorInsertVSVGmut
UseTagsExpressionMammalianMutationK47Q, R354APromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Ub KO.G76V
Plasmid#11932DepositorInsertUbiquitin KO.G76V (RBX1 Human)
UseTagsGFPExpressionMammalianMutationConjugation deficient mutant. All 7 lysines of ub…PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike-VLP
Plasmid#173922PurposeExpresses virus-like particles based upon the original SARS-Cov-2DepositorInsertsUseTagsno tags, native proteinExpressionBacterial and MammalianMutationPromoter22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…Available sinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-SARS-CoV-2-S (Wuhan)
Plasmid#215812PurposeExpresses SARS-CoV-2 Spike protein in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCRISPomyces-2BD
Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
L2383-FRB-GPA-NLuc
Plasmid#222902PurposePositive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.DepositorInsertFRB-GPA-20GS-NanoLuc
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
UseTagsExpressionBacterialMutationPromoterglnS' and lambda(ts) and proKAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT22-GluCNZ-6His
Plasmid#213504PurposeDesigned for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.DepositorInsertGlucanase (glucan endo-1,3-beta-glucosidase)
UseTags6His tagExpressionBacterialMutation1-36 Signal Sequence DeletionPromoterT7Available sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19/ER-mChe
Plasmid#183163PurposeConstruct for transient expression of endoplasmic reticulum-targeted mCherry in plants.DepositorInsertER-mCherry
UseTags25 aa ER signal of Glycine soja kunitz-type tryps…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HLA-A2_GFP
Plasmid#85161PurposeHLA.A2_GFP fusion with 2 glycines in betweenDepositorInsertHLA.A2 (HLA-A Human)
UseGateway entry vectorTagsEGFPExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKC52
Plasmid#226780PurposeExpresses Drosophila UNC-104(R348G) in insect cellsDepositorInsertDrosophila unc-104 (R348G) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Arginine 348 to GlycinePromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
YFP-Cdc42(V12)
Plasmid#11399DepositorInsertcell division cycle 42 (CDC42 Human)
UseTagsYFPExpressionMammalianMutationObtained with Glycine 12 changed to Valine.PromoterAvailable sinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RBD-JA1
Plasmid#167126PurposeExpresses SARS-CoV-2 RBD in bacterial systemDepositorInsertSARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
UseTagsExpressionBacterialMutationresidues (333 to 529 aa)PromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S (D614G)
Plasmid#215838PurposeExpresses SARS-CoV-2 Spike protein in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTRm
Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…TagsExpressionMutationPromoterNoneAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-YTB
Plasmid#26721DepositorInsertUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
p1A
Plasmid#162692PurposeExpresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOME0182
Plasmid#178403PurposeExpresses KSHV K8.1 glycoprotein hyp taggedDepositorInsertKSHV K8.1
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE476
Plasmid#153267PurposeM2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-c/o NL4-3 Env delta CT
Plasmid#139285PurposeHIV-1 NL4-3 glycoprotein lacking cytoplasmic tailDepositorInsertcodon optimized HIV-1 Env delta cytoplasmic tail
UseTagsExpressionMammalianMutationdeletion of cytoplasmic tail after residue 710PromoterCMV IEAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-NGLY1-Puro
Plasmid#181916PurposeLentiviral plasmid that directs the expression of NGLY1 in mammalian cells.DepositorInsertN-glycanase 1 (NGLY1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
T2A-mCherry (MTK 4a) (pAN3844)
Plasmid#194223PurposeMammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherryDepositorInsertT2A-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK-Cas9-gate
Plasmid#113743PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.DepositorInsertCas9
UseCRISPR; Gateway destination vectorTagsExpressionMutationPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-RexYFP
Plasmid#141070PurposeBacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratioDepositorInsertRexYFP
UseTags6xHisExpressionBacterialMutationchanged Leucine 169 to Proline, changed Tyrosine …PromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDGE657
Plasmid#153255PurposeM2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE479
Plasmid#153270PurposeM4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RQ/TG
Plasmid#25369DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationThreonine 1343 mutated to Glycine Arginine 139…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pED-hIRE1(I642G)
Plasmid#16263DepositorInsertIRE1 (ERN1 Human)
UseTagsExpressionMammalianMutationisoleucine to glycine mutation at residue 642PromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ub[9]-mRFP[1]
Plasmid#20249DepositorInsertubiquitin C (UBC Human)
UseTagsmRFPExpressionMammalianMutationchanged Stop codon 686 to GlycinePromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe neo TGFBR3-HA
Plasmid#83095PurposeRetroviral vector for constitutive TGFBR3 expression with C-terminal HA tagDepositorInsertTGFBR3 (TGFBR3 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mOtop1_pGEMHE
Plasmid#114674PurposeThis construct was used to express mouse Otop1 in Xenopus laevis oocytesDepositorInsertOtopetrin 1 (Otop1 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationSNP (rs49325632): Glycine 396 to AlaninePromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-R1441G
Plasmid#25082DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationArginine 1441 mutated to GlycinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only