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We narrowed to 14,379 results for: TIM

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Showing: 11161 - 11180 of 14379 results

  1. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  8. pJC30X-BS(X)

    Plasmid
    #58958
    Purpose
    Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.
    Insert
    Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
    Tags
    First 8 codons of COX3
    Mutation
    GFP modified as described: CORMACK, B. P., R. H. …
    Promoter
    n/a
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  13. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  19. pMLS791

    Plasmid
    #188889
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188889-plasmid-map-sequence-id-370713

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  20. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid
    #197952
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Expression
    Plant
    Promoter
    AtU6-26 and pUBQ10
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197952-plasmid-map-sequence-id-387870

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Showing: 11161 - 11180 of 14379 results