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Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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AP1297‐1
Plasmid#87247Purposegtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
UseTagsgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…ExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
UseTagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pYI277
Plasmid#228285PurposeConstitutive EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDM097
Plasmid#216819PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.DepositorInsertmStayGold
UseTagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…ExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7061
Plasmid#167230Purposecell-free cascaded lead sensorDepositorInsertpPbr-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA-pTRE-B
Plasmid#218894Purposegolden gate cloningDepositorInsertTRE
UseSynthetic BiologyTagsExpressionMutation580G>CPromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGF-WPRE-G
Plasmid#218901Purposegolden gate cloningDepositorInsertWPRE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGD_MUTT2A_E
Plasmid#218899Purposegolden gate cloningDepositorInsertMUTT2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGD-T2A-E
Plasmid#218898Purposegolden gate cloningDepositorInsertT2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p223-Pulse-Switch
Plasmid#217889PurposeLentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NK811
Plasmid#196408PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly controlDepositorInsertSro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP
Plasmid#188272PurposeMammalian expression, Genome editingDepositorInsertAaCas12b(Q119F/E475R/E758R)
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP
Plasmid#188274PurposeMammalian expression, Genome editingDepositorInsertPlmCasX(T26R/K610R/K808R)
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPG-GPD-TRPC
Plasmid#190113PurposeExpression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIPKAH
Plasmid#185869PurposeKnocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)DepositorInsertGPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only