We narrowed to 73,945 results for: LAS

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pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

Plasmid

#115142

Purpose

CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Mb3Cas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

Plasmid

#209781

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

cTNT

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

Plasmid

#166413

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

Protection Of Telomeres 1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

ΔOB deleted 125 a.a

Promoter

ef1-a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD hCPS1 AD

Plasmid

#188130

Purpose

Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST flag-CAD E1368A

Plasmid

#188141

Purpose

Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR

Plasmid

#48157

Purpose

Transgenically expresses firefly luciferase protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

luciferase

Use

Luciferase; T. cruzi expression

Promoter

T7

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

Plasmid

#205541

Purpose

Express EPN1-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

Plasmid

#205545

Purpose

Express EPN11-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1396A

Plasmid

#188126

Purpose

Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

Plasmid

#71219

Purpose

dCas9 fused to the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Use

Lentiviral

Tags

dSpCas9-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLK89 - Edit-directing plasmid cloning insert

Plasmid

#98846

Purpose

Contains the insert used for step 2 of edit-directed plasmid cloning

Depositor

Leonid Kruglyak

Insert

KanR

Use

CRISPR

Expression

Yeast

Mutation

Changed sequence to remove a MluI cut site

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 V235fs BLAST

Plasmid

#164236

Purpose

lentiviral plasmid for GFP-TREX1 V235fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

V235 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

Plasmid

#114090

Purpose

CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized MbCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-222-PGK-GFP-Blast

Plasmid

#31522

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-222 (Mir222 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192239

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192242

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192241

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

Plasmid

#139230

Purpose

mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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