166,169 results

1. EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH

   Plasmid

   #235294

   Purpose

   ComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sites

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-mitoLbNOX-Flag

   Plasmid

   #234985

   Purpose

   Expresses mitochondrial LbNOX in human cell lines

   Depositor

   Denis Titov

   Article

   Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

   Insert

   mito-LbNOX
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgMARK2-3-3

   Plasmid

   #229442

   Purpose

   knockout of MARK2 and MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-3-3 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

   Plasmid

   #223253

   Purpose

   AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

   Depositor

   Michael Do

   Article

   Liu et al Science. 2023 Jan 27;379(6630):376-381.

   Insert

   macaque OPN4-IRES-EGFP (OPN4 )
   

   Use

   AAV

   Promoter

   human Synapsin 1 (hSyn)

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-FKBP-OPHN1

   Plasmid

   #221310

   Purpose

   Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   OPHN1 (pLxIS) (Ophn1 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-TetON-mEGFP-MYOD1_WT

   Plasmid

   #215617

   Purpose

   For integration of MYOD1 WT T2A mEGFP

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   MYOD1 (MYOD1 Human)
   

   Tags

   T2A mEGFP

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-TetON-mEGFP-MYOD1_AroPERFECT-C

   Plasmid

   #215621

   Purpose

   For integration of MYOD1 AroPERFECT C T2A mEGFP

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   MYOD1 AroPERFECT C (MYOD1 Human)
   

   Tags

   T2A mEGFP

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHTC-HaloTag_SUZ12:1-739

   Plasmid

   #211010

   Purpose

   Mammalian expression of full-length human SUZ12. C-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   SUZ12:M1-L739 (SUZ12 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHTN-HaloTag_SUZ12:1-739

    Plasmid

    #211009

    Purpose

    Mammalian expression of full-length human SUZ12. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SUZ12:M1-L739 (SUZ12 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pJRA105

    Plasmid

    #209338

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal Dendra2 fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GOSR2(G144W)-mCherry

    Plasmid

    #209887

    Purpose

    Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells

    Depositor

    Geert van den Bogaart

    Insert

    GOSR2 (GOSR2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed glycine 144 to tryptophan; this mutation …

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ELMOD3-HA

    Plasmid

    #201902

    Purpose

    Used for expression of human protein ELMOD3 in mammalian cells

    Depositor

    Richard Kahn

    Insert

    ELMOD3 (ELMOD3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EMC1-2EGFP-ter-NAT

    Plasmid

    #184763

    Purpose

    Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    EMC1 (EMC1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-Spe3-RfA-Venus

    Plasmid

    #186372

    Purpose

    Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176575

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti sgRNA NGFR GFP out of frame

    Plasmid

    #155282

    Purpose

    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280

    Depositor

    Martin Carroll

    Insert

    GFP out of frame IRES NGFR
    

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW2610_pCAG-PV1-FKBP-L1-iCre-257C-NLS-BGHpA

    Plasmid

    #108736

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to FKBP CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FKBP and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJF5

    Plasmid

    #21679

    Depositor

    James Haber

    Article

    Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Mutation

    HO digestion site in one of the LacZ genes

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only