161,813 results

1. mCherry2-N1

   Plasmid

   #54517

   Purpose

   Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Neo-M2rtTA

   Plasmid

   #60843

   Purpose

   AAVS1 donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

   Inserts

   M2rtTA
   Neo
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-opEBNA2-IRES-GFP

   Plasmid

   #220354

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   3 x HA tag

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4X PRE TK luc

   Plasmid

   #206159

   Purpose

   Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   4X PRE TK luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. HA-HIF1alpha-wt-pBabe-puro

   Plasmid

   #19365

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt human cDNA

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SF-SON IRES Tomato

   Plasmid

   #214403

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NRF2

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAFNF-GFP

    Plasmid

    #13772

    Purpose

    Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDual_dsCas9_Blast

    Plasmid

    #214681

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LPC-flag-REST-WT

    Plasmid

    #41903

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer20

    Plasmid

    #44012

    Purpose

    Tet-inducible lentiviral vector for ORF expression

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQC-CherryOFF-GFP

    Plasmid

    #129101

    Purpose

    Reporter vector

    Depositor

    Fangliang Zhang

    Article

    Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

    Insert

    CherryOFF
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G-> A

    Promoter

    CMV

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiV2-hU6-evopreQ1

    Plasmid

    #210189

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

    Plasmid

    #188768

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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