Addgene

We narrowed to 2,075 results for: PAM

Showing: 1681 - 1710 of 2075 results

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PAS501 (TJF040)

Plasmid

#65567

Purpose

pETDuet-1 Containing FadD Q338R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with Q338R mutation

Use

Tags

Expression

Bacterial

Mutation

Q338R

Promoter

T7/lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS511 (TJF032 Y375R)

Plasmid

#65578

Purpose

pETDuet-1 Containing FadD Y375R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

Y375R

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS536 (TJF079)

Plasmid

#65610

Purpose

pETDuet-1 Containing FadD T349A-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

T349A

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS505 (TJF068)

Plasmid

#65572

Purpose

pETDuet-1 Containing FadD V451A

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with V451A mutation

Use

Tags

Expression

Bacterial

Mutation

V451A

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS517 (TJF084)

Plasmid

#65584

Purpose

pETDuet-1 Containing FadD F447D S379R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

F447D S379R

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS533 (TJF076)

Plasmid

#65607

Purpose

pETDuet-1 Containing FadD V451A-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

V451A

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS512 (TJF032 S377R)

Plasmid

#65579

Purpose

pETDuet-1 Containing FadD S377R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

S377R

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS510 (TJF032 G446S)

Plasmid

#65577

Purpose

pETDuet-1 Containing FadD G446S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

G446S

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pPS834

Plasmid

#8932

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pJK384.4

Plasmid

#155369

Purpose

dCas9 + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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FKBP-NTEVp(WT)

Plasmid

#137831

Purpose

Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-NTEVp(WT)

Use

Tags

3x FLAG and NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-CTEVp(WT)

Plasmid

#137832

Purpose

Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CTEVp(WT)

Use

Tags

3x FLAG and NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-PSMA

Plasmid

#11524

Depositor

Pamela Bjorkman

Article

Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

Insert

prostate-specific membrane antigen (FOLH1 Human)

Use

Tags

His

Expression

Insect

Mutation

There is a short N-terminal truncation of this pr…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Use

Tags

Expression

Bacterial

Mutation

copFnull

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Article

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

gfap

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-chlorR

Plasmid

#199992

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Use

Tags

Expression

Bacterial

Mutation

copFnull

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FRBl2-E

Plasmid

#15289

Depositor

David Spencer

Article

Li et al Gene Ther. 2002 Feb . 9(4):233-44.

Insert

FRAP-rapamycin binding domain (MTOR Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

res. 2025-2113, T2098L

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ2-Pdadh-LacZ

Plasmid

#191618

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-EEA1

Plasmid

#222419

Purpose

To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

EEA1 (EEA1 Human)

Use

Tags

FRB-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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PD29

Plasmid

#164542

Purpose

Expresses Drd1-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lentiviral-FKBP-cpGFP-Nb39

Plasmid

#191498

Purpose

Rapamycin-dependent opioid sensor for opioid receptors (component 2)

Depositor

Wenjing Wang

Article

Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

Insert

FKBP-cpGFP-Nb39

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-RapR-PTPPest

Plasmid

#188657

Purpose

Rapamycin regulated PTPPest with mVenus tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Protein Tyrosine Phosphatase Pest (PTPN12 )

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCB6-HFE-EGFP

Plasmid

#12104

Depositor

Pamela Bjorkman

Article

Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

Insert

HFE (HFE Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pJK506

Plasmid

#155382

Purpose

dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-GCaMP5g-P2A-Vmn1r58

Plasmid

#133418

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lentiviral-MOR-FRB

Plasmid

#191497

Purpose

Rapamycin-dependent opioid sensor for the mu opioid receptor (component 1)

Depositor

Wenjing Wang

Article

Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

Insert

MOR-FRB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lentiviral-KOR-FRB

Plasmid

#191496

Purpose

Rapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)

Depositor

Wenjing Wang

Article

Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

Insert

KOR-FRB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-NGFP-VMAn-FKBP

Plasmid

#70225

Purpose

Expresses N-terminal portion of GFP along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

eGFP

Use

Tags

FK506- and rapamycin-binding protein (FKBP), Link…

Expression

Mammalian

Mutation

Changed Glu 125 to Ile, Deleted amino acids 129-2…

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
Rap-MESA-20-M-Gal4

Plasmid

#84554

Purpose

MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FRB-20-M-Gal4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge