We narrowed to 2,701 results for: LRE
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Plasmid#221911PurposeTransient expression of GFP-tagged CBPKTG in mammalian cellsDepositorInsertCBP (CREBBP Human)
TagsGFPExpressionMammalianMutationmutation of lysine residues to glycine residues (…Available SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdCFIDdAL
Plasmid#221916PurposeTransient expression of GFP-tagged CBPdeltaCFIDdeltaAL in mammalian cellsDepositorInsertCBP (CREBBP Human)
TagsGFPExpressionMammalianMutationdeletion of the CBP-FUS interaction domain (CFID)…Available SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdIDR6KTG
Plasmid#221913PurposeTransient expression of GFP-tagged CBPdeltaIDR6KTG in mammalian cellsDepositorInsertCBP (CREBBP Human)
TagsGFPExpressionMammalianMutationdeletion of IDR6 (aa 1856 - 2090); mutation of ly…Available SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdIDR7KTG
Plasmid#221915PurposeTransient expression of GFP-tagged CBPdeltaIDR7KTG in mammalian cellsDepositorInsertCBP (CREBBP Human)
TagsGFPExpressionMammalianMutationdeletion of IDR7 (aa 2109-2434); mutation of lysi…Available SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304_miRFP670nano-P2A-ERM-gCam6f
Plasmid#214927PurposeExpress miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)DepositorInsertmiRFP670nano-P2A-ERM-gCam6f
UseLentiviralExpressionMammalianPromoterCMVpAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3291
Plasmid#144767PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPrdm9#1/Cre
Plasmid#193231PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 geneDepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPrdm9#2/Cre
Plasmid#193232PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 geneDepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgMroh2b#2/Cre
Plasmid#193220PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b geneDepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgMroh2b#1/Cre
Plasmid#193219PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b geneDepositorAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-POLB-KO-g1
Plasmid#176090PurposeCas9 plus POLB gRNA #1; contains a puromycin resistance cassetteDepositorAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E55A E57A K58A nsp1
Plasmid#175427PurposeExpresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E55A E57A K58A
Tags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E55A E57A K58APromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU002
Plasmid#174299PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
TagsHA and cMycExpressionYeastMutationN343Q, N501YAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU001
Plasmid#174298PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
TagsHA and cMycExpressionYeastMutationN343Q, E484KAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-P667A
Plasmid#133750PurposeEGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmidDepositorInsertMutant P667A EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianMutationproline 667 converted to alanine (Please see depo…PromoterEF1-alphaAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSico_U6-PGC1a1 sgRNA
Plasmid#165425PurposeExpression of gRNA against human PGC-1a variant 1DepositorInsertgRNA against PGC-1a variant 1 (PPARGC1A Synthetic, Human)
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-CD55-NeoR
Plasmid#153551PurposeUsed as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AMutationa 1-bp mutation in exon 4PromoterNo promoterAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA-NeoR
Plasmid#153549PurposeUsed as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-720aa)
Plasmid#139687PurposeExpresses N-teminal Flag tagged aa 1- 720 of LARS1DepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W
Plasmid#105022PurposeLentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W
Plasmid#105032PurposeLentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
ExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL5 sgRNA(MS2)-MS2-hA3a D131A
Plasmid#112131Purposeempty sgRNA cloning vector with MS2-humanA3a D131ADepositorInserthuman Apobec3a D131A mutant (APOBEC3A Human)
UseCRISPRExpressionMammalianMutationD131A mutant in hA3aPromoterhU6,CBhAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP
Plasmid#108503Purposeinducible expression of vsv-INCENP d543-746-EGFPDepositorInsertINCENP d543-746 (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationdelta alpha helixPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP
Plasmid#108505Purposeinducible expression of vsv-INCENP 8KR-EGFPDepositorInsertINCENP (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationK548R, K563R, K602R, K609R, K614R, K622R, K630R, …PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP
Plasmid#108506Purposeinducible expression of vsv-INCENP 8KQ-EGFPDepositorInsertINCENP (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationK548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDK11A gRNA (BRDN0001145793)
Plasmid#75889Purpose3rd generation lentiviral gRNA plasmid targeting human CDK11ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK11A gRNA (BRDN0001146824)
Plasmid#75890Purpose3rd generation lentiviral gRNA plasmid targeting human CDK11ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK11A gRNA (BRDN0001147032)
Plasmid#75891Purpose3rd generation lentiviral gRNA plasmid targeting human CDK11ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK11A gRNA (BRDN0001147530)
Plasmid#75892Purpose3rd generation lentiviral gRNA plasmid targeting human CDK11ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147L-siResist
Plasmid#49856PurposeEncodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationMethionine100, Methionine 125, and Methionine 147…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125L-siResist
Plasmid#49855PurposeEncodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationMethionine100 and Methionine 125 mutated to Leuci…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV_HyPer7RgDAAO
Plasmid#217653PurposeExpresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membraneDepositorInsertHyPer7 D amino acid oxidase
UseLentiviralTagsNuclear export signalExpressionMammalianMutationFused DAAO to the C-terminus of HyPer7 using a Gl…PromoterCMVAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Puro
Plasmid#211364PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
3X FLAG-FUS-WT
Plasmid#44985DepositorAvailable SinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W
Plasmid#67982PurposeCas9 activity reporter with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPREAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianPromoterCAGAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human Erbb2-IRES-GFP
Plasmid#91888PurposeRetroviral expression of human Erbb2DepositorAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only