We narrowed to 4,845 results for: phy

1. pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163043

   Purpose

   QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSW454 - pUFO

   Plasmid

   #115978

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Jan Lohmann

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pUFO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAD-eLACCO1.1

   Plasmid

   #167944

   Purpose

   Fluorescent biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTfR-PAmKate

   Plasmid

   #32692

   Depositor

   Vladislav Verkhusha

   Article

   Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

   Insert

   PAmKate
   

   Use

   Tags

   transferrin receptor (TfR)

   Expression

   Mammalian

   Mutation

   F84W/S148N/S165G compared to mKate (but numbering…

   Promoter

   CMV

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-HA-ERK2 WT

   Plasmid

   #8974

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dendra2-Actin-C-18

   Plasmid

   #57701

   Purpose

   Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

   Plasmid

   #104119

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   CheRiff-CFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin I

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DNA-PKcs N-terminal sgRNA

   Plasmid

   #207093

   Purpose

   pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AGCGGGACTCGGCGGCATGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Halo-DNAPKcs HRD

   Plasmid

   #207088

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTorPE-jREX-GECO1

    Plasmid

    #113941

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

    Depositor

    Thomas Hughes, Robert Campbell

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jREX-GECO1
    

    Use

    Tags

    6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163072

    Purpose

    Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dendra2-Golgi-7

    Plasmid

    #57722

    Purpose

    Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mKate1.31-pBAD

    Plasmid

    #54827

    Purpose

    Localization: Protein Expression Vector , Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and mKate1.31

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAAV-CamKIIa-moxBFP-WPRE

    Plasmid

    #194976

    Purpose

    AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hum sgk promoter F4R4

    Plasmid

    #83426

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

    Insert

    human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    sgk

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CROP_C9_Puro_HDAC2

    Plasmid

    #183298

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    HDAC2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBPGAL4.2::p65Uw

    Plasmid

    #26229

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4::p65 (RELA Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Human SERCA3 (pMT2)

    Plasmid

    #75189

    Purpose

    Mammalian expression of ATP2A3

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

    Insert

    ATP2A3 (ATP2A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MAC_C_LMTK3

    Plasmid

    #187773

    Purpose

    MAC-tagged gene expression of human LMTK3

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LMTK3 (LMTK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRRL-VinculinTS-I997A

    Plasmid

    #111832

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A) (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed vinculin isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR-PpU6P-sgRNA-L3L2

    Plasmid

    #113740

    Purpose

    Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-PpU6P-sgRNA-L1L4

    Plasmid

    #113736

    Purpose

    Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. VV229: GCaMP6f in fck

    Plasmid

    #58514

    Purpose

    Expresses GCaMP6f under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Tags

    C-terminal FCYENEV (ER export motif); this was no…

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSW181 - GR-LhG4

    Plasmid

    #115983

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSW610 - GR-LhG4_BD

    Plasmid

    #115992

    Purpose

    entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4_BD
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRU55

    Plasmid

    #167684

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-flex-oChIEF-citrine

    Plasmid

    #50973

    Purpose

    flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    inverted and inserted between two lox sites

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. H6hSDHA

    Plasmid

    #113045

    Purpose

    Hetero expression of His-tagged human SDHA

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    H6hSDHA (SDHA Human)
    

    Use

    Tags

    His6 (MGSSHHHHHHSQDP-)

    Expression

    Bacterial

    Mutation

    Insert begins at amino acid 43

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RNF168-Halo HRD

    Plasmid

    #207080

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mEos2-Calreticulin-N-10

    Plasmid

    #57353

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

34. CyTERM-mGFP

    Plasmid

    #62237

    Purpose

    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

    Depositor

    Erik Snapp

    Article

    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

    Insert

    CyTERM-mGFP
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    signal anchor of rabbit cytochrome p450 and monom…

    Promoter

    CMV

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMF-FU_TetO_GW_PPARG2

    Plasmid

    #193289

    Purpose

    Lentiviral construct that expresses a dox-inducible human PPARG2

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    PPARG2 (PPARG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRRL_U2AF1_S34Y_mCherry

    Plasmid

    #84018

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34Y

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Use

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CMV-CAR-GECO1

    Plasmid

    #45493

    Purpose

    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    CAR-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEPSA5Cas9syn2

    Plasmid

    #206963

    Purpose

    S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

    Depositor

    Daijie Chen

    Article

    Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge