We narrowed to 44,145 results for: gats

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HcKCR1_mCherry_pcDNA3.1

Plasmid

#177336

Purpose

Expresses KCR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKL004

Plasmid

#98920

Purpose

Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

GCaMP6f tethered to mRuby

Tags

His and mRuby3

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLX313 MSLN

Plasmid

#193617

Purpose

Expresses MSLN

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

MSLN (MSLN Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-U6:gRNA

Plasmid

#63157

Purpose

Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEN-X-A-ccdB-G-Z

Plasmid

#218892

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Article

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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YFN43

Plasmid

#89487

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

Depositor

Alejandro Ferrando

Article

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-N (aa 1-154)

Expression

Plant

Promoter

2x35S

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

Plasmid

#202025

Purpose

Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)

Expression

Bacterial

Mutation

GvpB S9G-R31L-R85L

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDOXOFF 15xGly

Plasmid

#211345

Purpose

DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-mCherry

Plasmid

#20938

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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HRV-3C Protease-mCherry

Plasmid

#243792

Purpose

Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HRV-3C protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8HOIP

Plasmid

#50015

Purpose

Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

ring finger protein 31 (RNF31 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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