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We narrowed to 30,799 results for: Eng

Showing: 2851 - 2900 of 30799 results

  2. pDIRECT_21D

    Plasmid
    #91131
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91131-plasmid-map-sequence-id-182255

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  5. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60343-plasmid-map-sequence-id-96621

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  9. PatoG-OG241

    Plasmid
    #72839
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoG
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72839-plasmid-map-sequence-id-133603

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  11. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  19. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

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  21. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185869-plasmid-map-sequence-id-370814

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  22. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

    Plasmid
    #127776
    Purpose
    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus
    Depositor
    Tatjana Sauka-Spengler
    Article
    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517
    Insert
    tfap2b (TFAP2B Chicken)
    Use
    Unspecified
    Tags
    Avi
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127776-plasmid-map-sequence-id-250021

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  23. PatoK-OG241

    Plasmid
    #72840
    Purpose
    NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoK
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoK
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72840-plasmid-map-sequence-id-133604

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  24. PatoL-LOG241

    Plasmid
    #72846
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoL
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72846-plasmid-map-sequence-id-133612

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  25. PatoK-LOG241

    Plasmid
    #72848
    Purpose
    NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoK
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoK
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72848-plasmid-map-sequence-id-133611

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  26. Gal4pBS134-Pleum

    Plasmid
    #60339
    Purpose
    Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    60339-plasmid-map-sequence-id-96620

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  43. pD3sE_SC12

    Plasmid
    #219720
    Purpose
    DV3 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV3 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219720-plasmid-map-sequence-id-433924

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  47. FUT8-Dox

    Plasmid
    #124631
    Purpose
    Doxycycline-inducible FUT8 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight and hEF1a
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124631-plasmid-map-sequence-id-248079

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  49. pYPQ166-SpRY

    Plasmid
    #161520
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9
    Use
    CRISPR; Gateway compatible zsprycas9 entry clone
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161520-plasmid-map-sequence-id-325640

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  50. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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Showing: 2851 - 2900 of 30799 results