159,557 results

1. pCDH-p18-CIBN

   Plasmid

   #188990

   Purpose

   A lentiviral plasmid encoding lysosome-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Use

   Tags

   p18

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-MyoAAV1E

   Plasmid

   #224439

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDTMSK insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn-PdCO-EGFP-WPRE

   Plasmid

   #198513

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN-Sth1Cas9

   Plasmid

   #221386

   Purpose

   Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   Sth1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MS2-HB

   Plasmid

   #35573

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   MS2 Coat Protein (cp Bacteriophage)
   

   Use

   Retroviral

   Tags

   HTBH

   Expression

   Mammalian

   Mutation

   Insert is a tail-to-head tandem placement of two …

   Promoter

   CMV

   Available since

   May 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2-H1-10

   Plasmid

   #57457

   Purpose

   Localization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   H1 (H1f0 Mouse)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHCMV-10A1

   Plasmid

   #15805

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   10A1 Murine leukemia virus envelope mRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-EB1

   Plasmid

   #17234

   Depositor

   Lynne Cassimeris

   Article

   Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

    Plasmid

    #39299

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. GCEPIA1-SNAPER

    Plasmid

    #172659

    Purpose

    Detect the real-time ER calcium store and local calcium microdomains in live cells.

    Depositor

    Pingyong Xu

    Article

    Luo et al Biophys Rep (2019) 5, 31–42

    Insert

    calmodulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-tetO-MCS

    Plasmid

    #84008

    Purpose

    doxycycline-inducible lentiviral vector

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

    Plasmid

    #115795

    Purpose

    Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrIIA4
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_219

    Plasmid

    #164559

    Purpose

    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Inserts

    First sgRNA cassette
    Second sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Dual sgrna

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and Mouse U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dendra2-CD9-10

    Plasmid

    #57705

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pLX301-ZIM3-KRAB-PYL1

    Plasmid

    #154761

    Purpose

    Expresses ZIM3 KRAB domain fused to PYL1

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3puro-mGFP

    Plasmid

    #225446

    Purpose

    Express mEGFP-fusion protein of interest

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. xPERT-hA2q

    Plasmid

    #231063

    Purpose

    Circularly permuted CasRx platform fused to human deaminase domain for A to I editing

    Depositor

    Meng How Tan

    Article

    Wang et al Nat Biotechnol. 2024 Oct 9. doi: 10.1038

    Inserts

    dCas13d
    Human deaminase ADAR2dd (ADARB1 Ruminococcus flavefaciens XPD3002)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H460D, E488Q, only the deaminase domain (aa 276-7…

    Promoter

    CMV

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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