162,326 results

1. pcDNA3.1-MS2-HA-HshnRNPC1_Z

   Plasmid

   #148114

   Purpose

   Mammalian Expression of HshnRNPC1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPC1 (HNRNPC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB-cT3G-cERP2-HDAC2

   Plasmid

   #222553

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   HDAC2 (HDAC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAK005

   Plasmid

   #233642

   Purpose

   For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   eSpCas9(1.1) with c-terminal VitelloTagB
   

   Use

   Tags

   6-His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUltra-hot

   Plasmid

   #24130

   Purpose

   3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

   Depositor

   Malcolm Moore

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pJAT10

   Plasmid

   #204293

   Purpose

   For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLAG-HA-FbxO5-pcDNA3.1-

   Plasmid

   #52509

   Purpose

   expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO5 (FBXO5 Human, FbxO5)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHCMV-SS-antiHAscFv-TM

   Plasmid

   #198302

   Purpose

   Displays anti-HA scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiHAscFv-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

   Plasmid

   #182377

   Purpose

   Dual expression construct encoding 3xFLAG and mCherry from separate promoters

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Inserts

   3xFlag
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSV TK promoter and SV40 promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ATP5F1B-pmCherry

    Plasmid

    #232783

    Purpose

    Encodes an mCherry-tagged ATP5F1B

    Depositor

    Giulia Bertolin

    Article

    Sharma et al Cell Death Discov. 2023 Jun 29;9(1):203.

    Insert

    ATP5F1B (ATP5F1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-GFP-Kir2.1

    Plasmid

    #107184

    Purpose

    mammalian expression of Kir2.1

    Depositor

    Bikash Pattnaik

    Insert

    KCNJ2 (KCNJ2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131462

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A K27M, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMV1 pEF1a-HGF

    Plasmid

    #188748

    Purpose

    Vector constitutively expressing HGF

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223507

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Insert

    GFP(Y39TAG)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Y39TAG

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-mSmo

    Plasmid

    #25395

    Depositor

    Philip Beachy

    Article

    Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

    Insert

    mouse Smoothened cDNA (Smo Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGBacmam-NGS Wnt-Fc

    Plasmid

    #159629

    Purpose

    Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7/8 subtype NGS Wnt Fc
    

    Use

    Tags

    Fc tag and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET22b-PelB-6H-Ag43-160N-sfGFP

    Plasmid

    #172886

    Purpose

    Display sfGFP protein on E. coli cell surface

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    SfGFP
    Ag43, partial (flu )
    

    Use

    Tags

    HHHHHH and TEV recognition site (ENLYFQG)

    Expression

    Bacterial

    Mutation

    Deleted 1-160 (N terminal)

    Promoter

    T7 Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOpen-TEV protease

    Plasmid

    #165509

    Purpose

    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    TEV protease
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge